Home Journals Articles Updates About
CN
image
收藏切换
Construction of efficient yeast cell factories for production of ginsenosides precursor dammarenediol-Ⅱ
收藏切换
PDF
Dong WANG1, 2, Yi LIU1, 2, Jiao-yang XU1, 2, Jin-he WANG1, 2, Zhu-bo DAI1, 2, *, Xue-li ZHANG1, 2, Lu-qi HUANG3
Acta Pharmaceutica Sinica | 2018, 53(8) : 1233 - 1241
Less
收藏切换
Acta Pharmaceutica Sinica | 2018, 53(8): 1233-1241
• SPECIAL REPORTS •
Construction of efficient yeast cell factories for production of ginsenosides precursor dammarenediol-Ⅱ
Full
Dong WANG1, 2, Yi LIU1, 2, Jiao-yang XU1, 2, Jin-he WANG1, 2, Zhu-bo DAI1, 2, *, Xue-li ZHANG1, 2, Lu-qi HUANG3
Affiliations
  • 1. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
  • 2. Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
  • 3. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
Published: 2018-08-12 doi: 10.16438/j.0513-4870.2018-0503
Outline
收藏切换

Dammarenediol-Ⅱ is an important precursor in the biosynthesis pathway of ginsenosides which are the main active components of Panax quinquefolius and Panax ginseng. For constructing a dammarenediol- Ⅱ-producing cell factory, the triterpenoid precursors of yeast are improved significantly by the modular pathway engineering strategy on the basis of an MVA optimized strain. The strain overexpressing Salvia miltiorrhiza SmFPS and Arabidopsis thaliana AtSQS2 could yield 67.4 mg·g-1 squalene, accounting for about 6.74% of cell dry weight. In our further work, an Arabidopsis thaliana 2, 3-oxidosqualene synthase AtSQE2 was found to be able to increase the downstream lanosterol yield by 22-fold, reaching 47.9 mg·g-1. Then, regulating dammarenediol-Ⅱ synthase gene expression, using anti-sense RNA technology for regulation of ERG7 in the ergosterol pathway, and optimizing fermentation process were successively performed. Finally, the synthesis flux of triterpenes was increased to 10 g·L-1 for the first time, and we constructed an efficient cell factory that can produce 15 g·L-1 dammarenediol-Ⅱ, which lays a solid foundation of industrial synthesis of dammarane-type ginsenosides.

ginsenosides  /  dammarenediol-Ⅱ  /  Sacchromyces cerevisiae  /  synthetic biology  /  microbial cell factories
Dong WANG, Yi LIU, Jiao-yang XU, Jin-he WANG, Zhu-bo DAI, Xue-li ZHANG, Lu-qi HUANG. Construction of efficient yeast cell factories for production of ginsenosides precursor dammarenediol-Ⅱ[J]. Acta Pharmaceutica Sinica, 2018 , 53 (8) : 1233 -1241 . DOI: 10.16438/j.0513-4870.2018-0503
Appendix
Less
Year 2018 volume 53 Issue 8
PDF
172
75
Cite this Article
BibTeX
Article Info
doi: 10.16438/j.0513-4870.2018-0503
  • Receive Date：2018-05-29
  • Online Date：2026-01-15
  • Published：2018-08-12
Article Data
Affiliations
History
  • Received：2018-05-29
  • Revised：2018-06-19
Funding
Affiliations
    1. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
    2. Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
    3. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
References
Share
https://castjournals.cast.org.cn/joweb/yxxb/EN/10.16438/j.0513-4870.2018-0503
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House