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The effects of TwHMGR overexpression on the biosynthesis of triptolide and celastrol in Tripterygium wilfordii
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Jia-dian WANG1, 2, Yu-jun ZHAO2, Yi-feng ZHANG1, 2, Tian-yuan HU1, Yun LU1, Jia-wei ZHOU1, Bao-wei MA1, Rui ZHANG1, Wei GAO1, *, Lu-qi HUANG2, *
Acta Pharmaceutica Sinica | 2018, 53(8) : 1225 - 1232
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Acta Pharmaceutica Sinica | 2018, 53(8): 1225-1232
• SPECIAL REPORTS •
The effects of TwHMGR overexpression on the biosynthesis of triptolide and celastrol in Tripterygium wilfordii
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Jia-dian WANG1, 2, Yu-jun ZHAO2, Yi-feng ZHANG1, 2, Tian-yuan HU1, Yun LU1, Jia-wei ZHOU1, Bao-wei MA1, Rui ZHANG1, Wei GAO1, *, Lu-qi HUANG2, *
Affiliations
  • 1. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
  • 2. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
Published: 2018-08-12 doi: 10.16438/j.0513-4870.2018-0431
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Tripterygium wilfordii 3-hydroxy-3-methylglutaryl coenzyme-A reductase (TwHMGR) is an important regulation site in terpenoids metabolic pathway in cytoplasm which is the first speed limit enzyme of MVA pathway. In order to investigate the effects of TwHMGR on the biosynthesis of triptolide and celastrol in Tripterygium wilfordii, the overexpression of TwHMGR (OE-HMGR) was studied in this paper. We cloned the full-length of TwHMGR to construct overexpression vector by Gateway technology then delivered the expression vector into Tripterygium wilfordii suspension cells by gene gun. qRT-PCR was used to detect the expression of TwHMGR:the expression of TwHMGR was increased to 1.75 folds over the control group (empty vector:pH7WG2D) in the overexpression group. The accumulation of triptolide and celastrol in the suspension cells of Tripterygium wilfordii was detected by UPLC, revealing that:the contents of triptolide and celastrol were increased to 163.93% and 190.04% of over the control group in the overexpression group. Based on these findings, the positive effect on the accumulation of active terpenoids, triptolide and celastrol in Tripterygium wilfordii was found and the results laid a foundation of the synthetic biology research on important active terpenoids in Tripterygium wilfordii.

TwHMGR  /  overexpression  /  triptolide  /  celastrol  /  gene gun
Jia-dian WANG, Yu-jun ZHAO, Yi-feng ZHANG, Tian-yuan HU, Yun LU, Jia-wei ZHOU, Bao-wei MA, Rui ZHANG, Wei GAO, Lu-qi HUANG. The effects of TwHMGR overexpression on the biosynthesis of triptolide and celastrol in Tripterygium wilfordii[J]. Acta Pharmaceutica Sinica, 2018 , 53 (8) : 1225 -1232 . DOI: 10.16438/j.0513-4870.2018-0431
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Year 2018 volume 53 Issue 8
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Article Info
doi: 10.16438/j.0513-4870.2018-0431
  • Receive Date：2018-05-09
  • Online Date：2026-01-15
  • Published：2018-08-12
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History
  • Received：2018-05-09
  • Revised：2018-06-11
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Affiliations
    1. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
    2. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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