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Expression analysis of Hsp70-related miRNA in Lonicera japonica Thunb. during different flowering stages
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Feng-jie ZHU1, 2, Juan LIU2, Yuan YUAN2, *, Jun-hui ZHOU2, Nian-jun YU1, *, Lu-qi HUANG2
Acta Pharmaceutica Sinica | 2018, 53(8) : 1202 - 1208
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Acta Pharmaceutica Sinica | 2018, 53(8): 1202-1208
• SPECIAL REPORTS •
Expression analysis of Hsp70-related miRNA in Lonicera japonica Thunb. during different flowering stages
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Feng-jie ZHU1, 2, Juan LIU2, Yuan YUAN2, *, Jun-hui ZHOU2, Nian-jun YU1, *, Lu-qi HUANG2
Affiliations
  • 1. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
  • 2. National Resource Center for Chinese Meteria Medica, China Academy of Chinese Medicine Sciences, Beijing 100700, China
Published: 2018-08-12 doi: 10.16438/j.0513-4870.2018-0389
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Plant flowering regulation is an important mechanism to response to environmental stress. Heat shock protein 70 family is one of the main molecular chaperones to resist stress; miRNA can be used as a negative regulator to participate in post-transcriptional gene in flowering network. In this paper, we obtained an Hsp70 gene from Lonicera japonica transcriptome and combined with Lonicera japonica miRNA library to obtain a novel miRNA that may target Hsp70 gene through bioinformatics method. Bioinformatics and expression during different flowering stages of the obtained Hsp70 gene and miRNA were analyzed. Phylogenetic tree showed that the obtained Hsp70 gene was clustered with Hsp110 subfamily in Oryza sativa and Arabidopis thaliana. The prediction of miRNA secondary structure showed its stable structure and high reliability. The binding site map showed that there were two base mismatches between sequences of miRNA and Hsp70 gene. The expression analysis showed that the expression of Hsp70 and miRNA in different flowering stages had opposite trends, indicating that miRNA might regulate Hsp70 to participate in the flowering stages of Lonicera japonica. This study provided new ideas for Lonicera japonica flowering regulation and response to environmental stress mechanisms.

Lonicera japonica  /  miRNA  /  Heat shock protein 70  /  qRT-PCR
Feng-jie ZHU, Juan LIU, Yuan YUAN, Jun-hui ZHOU, Nian-jun YU, Lu-qi HUANG. Expression analysis of Hsp70-related miRNA in Lonicera japonica Thunb. during different flowering stages[J]. Acta Pharmaceutica Sinica, 2018 , 53 (8) : 1202 -1208 . DOI: 10.16438/j.0513-4870.2018-0389
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Article Info
doi: 10.16438/j.0513-4870.2018-0389
  • Receive Date：2018-04-27
  • Online Date：2026-01-15
  • Published：2018-08-12
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  • Received：2018-04-27
  • Revised：2018-06-12
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    1. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
    2. National Resource Center for Chinese Meteria Medica, China Academy of Chinese Medicine Sciences, Beijing 100700, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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