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Effect on biosynthesis of terpenoid active components through RNA interference with MCT gene in Tripterygium wilfordii
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Ya-di SONG1, Yu-jun ZHAO2, Shang CHEN1, Tian-yuan HU1, Rui ZHANG1, Jia-dian WANG1, Yun LU1, Xiu-juan WANG1, 3, Wei GAO1, 3, *, Lu-qi HUANG2, *
Acta Pharmaceutica Sinica | 2018, 53(8) : 1209 - 1214
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Acta Pharmaceutica Sinica | 2018, 53(8): 1209-1214
• SPECIAL REPORTS •
Effect on biosynthesis of terpenoid active components through RNA interference with MCT gene in Tripterygium wilfordii
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Ya-di SONG1, Yu-jun ZHAO2, Shang CHEN1, Tian-yuan HU1, Rui ZHANG1, Jia-dian WANG1, Yun LU1, Xiu-juan WANG1, 3, Wei GAO1, 3, *, Lu-qi HUANG2, *
Affiliations
  • 1. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
  • 2. State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • 3. Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
Published: 2018-08-12 doi: 10.16438/j.0513-4870.2018-0397
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MCT is an important key enzyme in the terpenoid biosynthesis in MEP pathway. In this study, Gateway technology was used to construct RNAi vector of TwMCT, and a vector fragment with a size of 484 bp was obtained. The TwMCT RNAi vector was transferred into the suspension cells of Tripterygium wilfordii by gene gun. Accumulation of terpenoids was assayed by UPLC, and the result showed that the content of triptolide and celastrol in cells decreased by 23.4% and 42.8%, respectively, compared with the control group pK7GWIWG2D. Moreover, the gene expression of TwMCT and major genes in terpenoid biosynthesis pathway was detected by qRT-PCR, which demonstrated that the expression of TwMCT reduced by 29.2% relative to that of the control group pK7GWIWG2D, and the relative expression of TwDXR, TwGGPS, TwHMGR and TwHMGS diminished by 36.3%, 31.3%, 62.2%, and 29.1%, respectively, but the expression of TwDXS was up-regulated by 114.2%, and there was no significant change in TwFPS. Thus, it was verified in vivo that interference with TwMCT expression significantly inhibited the accumulation of triptolide and celastrol in Tripterygium wilfordii, laying a foundation for further exploring the regulation mechanism of MCT gene on the terpenoid biosynthesis in Tripterygium wilfordii.

Tripterygium wilfordii  /  TwMCT  /  RNAi  /  triptolide  /  celastrol
Ya-di SONG, Yu-jun ZHAO, Shang CHEN, Tian-yuan HU, Rui ZHANG, Jia-dian WANG, Yun LU, Xiu-juan WANG, Wei GAO, Lu-qi HUANG. Effect on biosynthesis of terpenoid active components through RNA interference with MCT gene in Tripterygium wilfordii[J]. Acta Pharmaceutica Sinica, 2018 , 53 (8) : 1209 -1214 . DOI: 10.16438/j.0513-4870.2018-0397
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doi: 10.16438/j.0513-4870.2018-0397
  • Receive Date：2018-05-02
  • Online Date：2026-01-15
  • Published：2018-08-12
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  • Received：2018-05-02
  • Revised：2018-05-09
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Affiliations
    1. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
    2. State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
    3. Beijing Key Lab of TCM Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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