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The mechanisms of paeonol reversing multidrug resistance in ovarian cancer SKOV3/DDP cells
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Li HAN1, 2, *, Xiao-juan GUO1, 2, Zhong CHEN3, Hua BIAN1, 2, *, Chao-yun ZHANG1, 2, Wen-hua ZANG1, 2, Qian WANG1, 2, Jiu-lue HU1, 2
Acta Pharmaceutica Sinica | 2018, 53(9) : 1511 - 1517
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Acta Pharmaceutica Sinica | 2018, 53(9): 1511-1517
• ORIGINAL ARTICLES •
The mechanisms of paeonol reversing multidrug resistance in ovarian cancer SKOV3/DDP cells
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Li HAN1, 2, *, Xiao-juan GUO1, 2, Zhong CHEN3, Hua BIAN1, 2, *, Chao-yun ZHANG1, 2, Wen-hua ZANG1, 2, Qian WANG1, 2, Jiu-lue HU1, 2
Affiliations
  • 1. Zhang Zhongjing College of Chinese Medicine, Nanyang Institute of Technology, Nanyang 473004, China
  • 2. Henan Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation, Nanyang Institute of Technology, Nanyang 473004, China
  • 3. College of Pharmaceutical Science, Soochow University, Suzhou 215123, China
Published: 2018-09-12 doi: 10.16438/j.0513-4870.2018-0407
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The purpose of this research is to investigate the effects and mechanisms of paeonol (PL), a phenolic compound found in many traditional Chinese formulations, on reversing drug resistance in the ovarian cancer resistant SKOV3/DDP cells. The results showed that PL had significant drug-resistant reversal effect on SKOV3/DDP cells. Flow cytometry showed that PL could inhibit P-glycoprotein (P-gp) function in a concentration-dependent manner. Fluorescent quantitative PCR and cell immunofluorescence techniques were used to detect mechanisms of action. Results revealed that both the inhibitory effect on MDR1/P-gp and metadherin (MTDH) expression and the induction effect on phosphatase and tensin homolog (PTEN), by 15, 30, and 60 μmol·L-1 PL, were increased with increased concentrations of PL (P < 0.01, P < 0.05). The inhibitory effect on MTDH mRNA and the induction effect on PTEN mRNA, by PI3K inhibitor LY294002, were stronger or equivalent to that of the 60 μmol·L-1 PL treated group; however, the inhibition or induction effect on MTDH or PTEN protein were only comparable to the 15 μmol·L-1 PL treated group. The present study shows that the effect of PL on SKOV3/DDP cells may be related to the inhibition of P-gp function and expression, the inhibition of MDR1, MTDH expression, and the induction of PTEN expression, all which can provide a theoretical foundation for PL as a drug resistance reversal agent on the treatment of ovarian cancer chemotherapy resistance.

paeonol  /  ovarian cancer  /  multidrug resistance  /  metadherin  /  phosphatase and tensin homolog
Li HAN, Xiao-juan GUO, Zhong CHEN, Hua BIAN, Chao-yun ZHANG, Wen-hua ZANG, Qian WANG, Jiu-lue HU. The mechanisms of paeonol reversing multidrug resistance in ovarian cancer SKOV3/DDP cells[J]. Acta Pharmaceutica Sinica, 2018 , 53 (9) : 1511 -1517 . DOI: 10.16438/j.0513-4870.2018-0407
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Article Info
doi: 10.16438/j.0513-4870.2018-0407
  • Receive Date：2018-05-02
  • Online Date：2026-01-15
  • Published：2018-09-12
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History
  • Received：2018-05-02
  • Revised：2018-06-06
Funding
Affiliations
    1. Zhang Zhongjing College of Chinese Medicine, Nanyang Institute of Technology, Nanyang 473004, China
    2. Henan Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation, Nanyang Institute of Technology, Nanyang 473004, China
    3. College of Pharmaceutical Science, Soochow University, Suzhou 215123, China
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表12种不同金属材料的力学参数
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Family		 属数
Number of
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Number of
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Percentage of
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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