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Inhibitory effect and mechanism of licochalcone A on NLRP3 inflammasome
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Shu-bin FU1, 2, Guang XU2, Yuan GAO3, Zhi-lei WANG4, Nan QIN1, Hui-min LIU2, Zhao-fang BAI2, *, Xiao-he XIAO2, *
Acta Pharmaceutica Sinica | 2018, 53(12) : 2050 - 2056
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Acta Pharmaceutica Sinica | 2018, 53(12): 2050-2056
• Pharmacology •
Inhibitory effect and mechanism of licochalcone A on NLRP3 inflammasome
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Shu-bin FU1, 2, Guang XU2, Yuan GAO3, Zhi-lei WANG4, Nan QIN1, Hui-min LIU2, Zhao-fang BAI2, *, Xiao-he XIAO2, *
Affiliations
  • 1. School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
  • 2. China Military Institute of Chinese Medicine, 302 Military Hospital of China, Beijing 100039, China
  • 3. School of Pharmacy, Beijing University of Traditional Chinese Medicine, Beijing 100029, China
  • 4. School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
Published: 2018-12-12 doi: 10.16438/j.0513-4870.2018-0713
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Lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs), treated with licochalcone A (LCA) and retreated with inflammasome inducers respectively (ATP and nigericin), were used to construct the inflammasome model of NLRP3 (NOD-like receptor family, pyrin domain containing 3), to investigate the inhibitory effect and the molecular mechanism of LCA on the activity of NLRP3 inflammasome. The secretion of mature interleukin (IL)-1β, tumor necrosis factor-α (TNF-α) and caspase-1 in the supernatants were analyzed by ELISA and the Caspase-Glo® 1 Inflammasome Assay. Supernatants and cell lysates were analyzed for the expression of pro-caspase-1, pro-IL-1β, ASC, NLRP3, IL-1β, caspase-1 by immunoblotting. The study shows that LCA inhibited the activity of caspase-1 and the secretion of IL-1β, and suppressed the activity of NLRP3 inflammasome. There was also slight inhibition of NLRC4 inflammasome induced by Lfn-Flic, but no effect on poly(dA:dT)-induced the absent in melanoma 2 (AIM2) inflammasome. Western blot showed that LCA had no effect on the protein expression of NLRP3 and pro-IL-1β, which was mediated by NF-κB pathway. In summary, LCA can inhibit the cleavage of pro-caspase-1 and suppress the secretion of IL-1β to reduce the inflammation response. The study was carried out under the approval of the Scientific Investigation Board of 302 Hospital of PLA.

licochalcone A  /  NLRP3 inflammasome  /  caspase-1  /  IL-1β
Shu-bin FU, Guang XU, Yuan GAO, Zhi-lei WANG, Nan QIN, Hui-min LIU, Zhao-fang BAI, Xiao-he XIAO. Inhibitory effect and mechanism of licochalcone A on NLRP3 inflammasome[J]. Acta Pharmaceutica Sinica, 2018 , 53 (12) : 2050 -2056 . DOI: 10.16438/j.0513-4870.2018-0713
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Article Info
doi: 10.16438/j.0513-4870.2018-0713
  • Receive Date：2018-08-06
  • Online Date：2026-01-15
  • Published：2018-12-12
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History
  • Received：2018-08-06
  • Revised：2018-09-02
Funding
Affiliations
    1. School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
    2. China Military Institute of Chinese Medicine, 302 Military Hospital of China, Beijing 100039, China
    3. School of Pharmacy, Beijing University of Traditional Chinese Medicine, Beijing 100029, China
    4. School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
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表12种不同金属材料的力学参数
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Number of
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Number of
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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