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Analysis of Folium Epimedium toxicity in combination with Radix Morindae Officinalis based on zebrafish toxicity/metabolism synchronization
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Jie LING1, 2, Mo WANG3, Ying CHEN1, 2, Jie SONG1, 2, E SUN1, 2, Zi-qi SHI1, 2, Liang FENG1, 2, Xiao-bin JIA1, 2, Ying-jie WEI1, 2, *
Acta Pharmaceutica Sinica | 2018, 53(1) : 74 - 83
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Acta Pharmaceutica Sinica | 2018, 53(1): 74-83
• ORIGINAL ARTICLES •
Analysis of Folium Epimedium toxicity in combination with Radix Morindae Officinalis based on zebrafish toxicity/metabolism synchronization
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Jie LING1, 2, Mo WANG3, Ying CHEN1, 2, Jie SONG1, 2, E SUN1, 2, Zi-qi SHI1, 2, Liang FENG1, 2, Xiao-bin JIA1, 2, Ying-jie WEI1, 2, *
Affiliations
  • 1. The Third Clinical School of Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China
  • 2. Key Laboratory of New Drug Delivery System of Chinese Materia Medica of State Administration of Traditional Chinese Medicine, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China
  • 3. The Liberation Army 93318 Army Hospital, Kaiyuan 112300, China
Published: 2018-01-12 doi: 10.16438/j.0513-4870.2017-0756
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Metabolic transformation in vivo is a critical approach in the study of toxicity, but real-time dynamic observation of the transformation is difficult. We proposed that zebrafish toxicity/metabolism synchronization may be used in the analysis of toxicity of Folium Epimedium (Yinyanghuo for Chinese, YYH) and the toxicity may be reduced by Radix Morindae Officinalis (Bajitian for Chinese, BJT). Healthy zebrafish embryos 1 day post fertilization (1 dpf) were exposed to different concentrations of YYH, total flavonoids of YYH (YTF), representative flavonoids (epimedin C and icariin) and their respective in combination with BJT. Death numbers of the embryos or larvals were counted during 1-5 days after dosing (2-6 dpf); embryonic micro-morphology of zebrafish (3 dpf) was observed and pictures were taken. The blank vehicle (0.4% DMSO) was used in the control group, and LC50 value of 2 to 6 dpf was calculated by SPSS16.0. A relative safe concentration was sampled every day to analyze the dynamic metabolites changes of major flavonoids of YYH. The results showed that epimedin A/B/C (EA/EB/EC) and icariin, the major flavonoids of YYH, were dynamically transformed into major metabolites of sagittatoside C (SC) and baohuoside I (BI) by zebrafish. BI was mainly derived from EA, EB and icariin. Neither original form nor their metabolite BI can cause zebrafish poisoning. SC was mainly derived from EC, and its accumulation was closely related to the toxicity of YYH, YTF and EC. After combination with BJT, the metabolism of EC was slowed down and the toxicity was alleviated. Zebrafish toxicity/metabolism synchronization revealed that the toxicity of EC of YYH was increased after metabolism into SC, which maybe the key potential poisonous factor of YYH, and BJT can reduce the toxicity by slowing down the metabolism rate of EC. The data provides new ideas and methods in the study of toxic substances in Chinese medicine and mechanism of detoxicity by combination.

zebrafish  /  Folium Epimedium  /  Radix Morindae Officinalis  /  toxicity  /  metabolism
Jie LING, Mo WANG, Ying CHEN, Jie SONG, E SUN, Zi-qi SHI, Liang FENG, Xiao-bin JIA, Ying-jie WEI. Analysis of Folium Epimedium toxicity in combination with Radix Morindae Officinalis based on zebrafish toxicity/metabolism synchronization[J]. Acta Pharmaceutica Sinica, 2018 , 53 (1) : 74 -83 . DOI: 10.16438/j.0513-4870.2017-0756
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Article Info
doi: 10.16438/j.0513-4870.2017-0756
  • Receive Date：2017-07-31
  • Online Date：2026-01-15
  • Published：2018-01-12
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History
  • Received：2017-07-31
  • Revised：2017-09-30
Funding
Affiliations
    1. The Third Clinical School of Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China
    2. Key Laboratory of New Drug Delivery System of Chinese Materia Medica of State Administration of Traditional Chinese Medicine, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China
    3. The Liberation Army 93318 Army Hospital, Kaiyuan 112300, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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