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Establishment of in-vitro model of complement activation and its application
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Cai-xia FENG1, Xian XIU2, Wei TIAN3, Yu-ling WANG1, Hong-ye WANG2, Ya-juan WANG2, *
Acta Pharmaceutica Sinica | 2017, 52(5) : 722 - 728
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Acta Pharmaceutica Sinica | 2017, 52(5): 722-728
• ORIGINAL ARTICLES Pharmacology •
Establishment of in-vitro model of complement activation and its application
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Cai-xia FENG1, Xian XIU2, Wei TIAN3, Yu-ling WANG1, Hong-ye WANG2, Ya-juan WANG2, *
Affiliations
  • 1. The Fifth Hospital of Shijiazhuang, Shijiazhuang 050021, China
  • 2. CSPC ZhongQi Pharmaceutical Technology (Shijiazhuang) Co., Ltd., Shijiazhuang 050035, China
  • 3. Hebei University of Chinese Medicine, Shijiazhuang 050200, China
Published: 2017-05-12 doi: 10.16438/j.0513-4870.2016-1107
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Complement activation-related pseudo-allergic reactions (CARPA) may represent 77% of all immune-mediated immediate hypersensitivity reactions. Because of the universality of the CARPA response and correlation between it and drug properties, complement activity tests are recommended as one of the tests for immunotoxicity and bioequivalence of drugs. However, in-vivo tests of complement activation are complicated, and the immunological differences between different individuals and between human and animal, making it very necessary to establish a standard and sample evaluation model for testing the effects of drugs on complement activity. In this study, the standard reaction serum was prepared by pooling sera collected from 40 healthy blood donors; a standard positive control was prepared by incubation with a heat-agglutinated IgG and zymosan A; SC5b-9, C5a, C4d and Bb were chosen as the test targets and evaluation criteria of the results was defined, all of these constituted the in-vitro model. By using this in-vitro model, the immunological toxicity of the different prescription of antifungal drug amphotericin B, and voriconazole for injection, and the bioequivalence of amphotericin B liposome formulations were studied.

hypersensitivity reaction  /  complement  /  in-vitro model  /  amphotericin B  /  voriconazole
Cai-xia FENG, Xian XIU, Wei TIAN, Yu-ling WANG, Hong-ye WANG, Ya-juan WANG. Establishment of in-vitro model of complement activation and its application[J]. Acta Pharmaceutica Sinica, 2017 , 52 (5) : 722 -728 . DOI: 10.16438/j.0513-4870.2016-1107
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Article Info
doi: 10.16438/j.0513-4870.2016-1107
  • Receive Date：2016-11-15
  • Online Date：2026-01-14
  • Published：2017-05-12
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History
  • Received：2016-11-15
  • Revised：2016-12-27
Funding
Affiliations
    1. The Fifth Hospital of Shijiazhuang, Shijiazhuang 050021, China
    2. CSPC ZhongQi Pharmaceutical Technology (Shijiazhuang) Co., Ltd., Shijiazhuang 050035, China
    3. Hebei University of Chinese Medicine, Shijiazhuang 050200, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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