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2, 3, 5, 4'-Tetrahydroxystilbene-2-O-β-D-glycoside attenuates the apoptotic responses of hypoxia/reoxygenation injury in bronchial epithelial cells through MAPK, HIF-1α and p53 pathway
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Pu-qiao LIAN1, Yan-nan FAN1, Hui YANG1, Li-xia FU1, Yun-xiao LI2, Qi HOU1, *
Acta Pharmaceutica Sinica | 2017, 52(7) : 1122 - 1132
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Acta Pharmaceutica Sinica | 2017, 52(7): 1122-1132
• ORIGINAL ARTICLES •
2, 3, 5, 4'-Tetrahydroxystilbene-2-O-β-D-glycoside attenuates the apoptotic responses of hypoxia/reoxygenation injury in bronchial epithelial cells through MAPK, HIF-1α and p53 pathway
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Pu-qiao LIAN1, Yan-nan FAN1, Hui YANG1, Li-xia FU1, Yun-xiao LI2, Qi HOU1, *
Affiliations
  • 1. State Key Laboratory of Natural Products and Functions, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
  • 2. Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
Published: 2017-07-12 doi: 10.16438/j.0513-4870.2017-0443
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This study was designed to investigate the effect of 2, 3, 5, 4'-tetrahydroxystilbene-2-O-β-D-glycoside(TSG)on hypoxia/reoxygenation(H/R)-induced oxidative stress injury and its potential mechanism in human bronchial epithelial cell(BEAS-2B)cells. BEAS-2B cells were exposed to H/R treatment. Level of intracellular ROS was detected using DCFH-DA probe and fluorescence microplate reader. Production of MDA and activity of SOD were evaluated with MDA and SOD kits. Nucleus was shaped by DAPI staining. Translocation of Bax to mitochondria was observed in MCF-7/GFP-Bax cells. Change in mitochondrial membrane potential was detected by JC-1 staining. Release of cytochrome C from mitochondria was detected by immunofluorescence. Expressions of mitochondrial/cytoplasmic Bax and cytochrome C, caspase-9, caspase-3, phosphorylated MAPK, HIF-1α and phosphorylated p53(p-p53) were determined by Western blotting. TSG significantly improved cell viability and reduced H/R-induced ROS production in BEAS-2B cells, while significantly decreased MDA production. It inhibited Bax translocation and nucleus fracture, reversed the decrease in mitochondrial membrane potential and inhibited the release of cytochrome C and following activation of caspase-9/caspase-3. Simultaneously, TSG down-regulated the signals of SAPK JNK1/2 and p38 MAPK without an impact in ERK1/2. It attenuated expression of HIF-1α and phosphorylation of p53. This study suggests that TSG could protect BEAS-2B against H/R-induced apoptosis, perhaps through the MAPK, HIF-1α and p53 pathways.

2, 3, 5, 4'-tetrahydroxystilbene-2-O-β-D-glycoside  /  hypoxia/reoxygenation  /  BEAS-2B cell  /  apop tosis  /  mitogen-activated protein kinase  /  hypoxia inducible factor-1α  /  p53
Pu-qiao LIAN, Yan-nan FAN, Hui YANG, Li-xia FU, Yun-xiao LI, Qi HOU. 2, 3, 5, 4'-Tetrahydroxystilbene-2-O-β-D-glycoside attenuates the apoptotic responses of hypoxia/reoxygenation injury in bronchial epithelial cells through MAPK, HIF-1α and p53 pathway[J]. Acta Pharmaceutica Sinica, 2017 , 52 (7) : 1122 -1132 . DOI: 10.16438/j.0513-4870.2017-0443
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Year 2017 volume 52 Issue 7
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Article Info
doi: 10.16438/j.0513-4870.2017-0443
  • Receive Date：2017-05-05
  • Online Date：2026-01-14
  • Published：2017-07-12
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History
  • Received：2017-05-05
  • Revised：2017-05-31
Affiliations
    1. State Key Laboratory of Natural Products and Functions, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
    2. Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
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表12种不同金属材料的力学参数
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小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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