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GC-MS separation and determination of 20 amino acids in biological samples based on ethyl chloroformate second derivation
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Gui-zhen WANG1, 2, 3, Xia HAO1, 2, 3, Lei LIU1, 2, 3, Zhen-yu LI1, 3, Xue-mei QIN1, 3, Guan-hua DU4, Xiang ZHANG5, Ke LI1, 3, *
Acta Pharmaceutica Sinica | 2017, 52(4) : 615 - 619
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Acta Pharmaceutica Sinica | 2017, 52(4): 615-619
GC-MS separation and determination of 20 amino acids in biological samples based on ethyl chloroformate second derivation
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Gui-zhen WANG1, 2, 3, Xia HAO1, 2, 3, Lei LIU1, 2, 3, Zhen-yu LI1, 3, Xue-mei QIN1, 3, Guan-hua DU4, Xiang ZHANG5, Ke LI1, 3, *
Affiliations
  • 1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
  • 2. College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China
  • 3. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China
  • 4. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
  • 5. University of Louisville, Louisville 40292, USA
Published: 2017-04-12 doi: 10.16438/j.0513-4870.2016-1028
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Due to the difference in structure and chemical properties of amino acids, it is difficult to determine amino acids with different properties by GC-MS. A method was established for the simultaneous determination of 20 amino acids by using ethyl chloroformate (ECF) as the derivatization reagent and adjusting pH 9-10 in the second derivatization in this study. The results showed that 20 amino acids were well separated and the compounds in the 2-20 μg·mL-1 concentrations were detected with a good linear response correlation coefficient r2 greater than 0.99. The results of precision and system adaptability show that RSD < 10%, the average recovery of samples in the 78.3%-109.9%. The results showed that the method was simple and easy to use, and the derivatization method could be carried out in aqueous solution. The derivatization product was stable with wide linear range, thus this method could be used for the determination of a variety of biological samples such as Astragalus mongolicus, mouse urine sample and mouse serum sample.

ethyl chloroformate derivatization  /  amino acid  /  GC-MS  /  separation  /  determination
Gui-zhen WANG, Xia HAO, Lei LIU, Zhen-yu LI, Xue-mei QIN, Guan-hua DU, Xiang ZHANG, Ke LI. GC-MS separation and determination of 20 amino acids in biological samples based on ethyl chloroformate second derivation[J]. Acta Pharmaceutica Sinica, 2017 , 52 (4) : 615 -619 . DOI: 10.16438/j.0513-4870.2016-1028
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Article Info
doi: 10.16438/j.0513-4870.2016-1028
  • Receive Date：2016-10-20
  • Online Date：2026-01-14
  • Published：2017-04-12
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History
  • Received：2016-10-20
  • Revised：2016-12-26
Funding
Affiliations
    1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
    2. College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China
    3. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China
    4. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
    5. University of Louisville, Louisville 40292, USA
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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