Home Journals Articles Updates About
CN
image
收藏切换
Chromatographic fingerprint analysis of Toosendan Fructus by HPLC-CAD coupled with chemometrics methods
收藏切换
PDF
Chun-ni ZHANG1, Ying-zi WANG1, *, Xin-guang SUN2, Yang ZHAO2, Wei ZHENG3, Wen-hua LI1, Zhen LONG4, Bai-ping MA2, *
Acta Pharmaceutica Sinica | 2017, 52(3) : 456 - 461
Less
收藏切换
Acta Pharmaceutica Sinica | 2017, 52(3): 456-461
Chromatographic fingerprint analysis of Toosendan Fructus by HPLC-CAD coupled with chemometrics methods
Full
Chun-ni ZHANG1, Ying-zi WANG1, *, Xin-guang SUN2, Yang ZHAO2, Wei ZHENG3, Wen-hua LI1, Zhen LONG4, Bai-ping MA2, *
Affiliations
  • 1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
  • 2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China
  • 3. Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
  • 4. ThermoFisher Scientific Corporation, Beijing 100080, China
Published: 2017-03-12 doi: 10.16438/j.0513-4870.2016-0965
Outline
收藏切换

A new method was developed for the chromatographic fingerprint analysis of Toosendan Fructus by HPLC coupled with the charged aerosol detector (CAD) in the present study. Samples were well separated on an Agilent ZOBAX SB C18 column (4.6 mm×250 mm, 5 μm) by gradient elution using acetonitrile and water containing 0.1% formic acid (v/v) at the flow rate of 1.0 mL·min-1. The column temperature was 30℃ and the injection volume was 5 μL. The nitrogen inlet pressure of the charged aerosol detector (CAD) was 35 psi, and the nebulizer chamber temperature was 35℃. In addition, the method of the chromatographic fingerprints combined with multivariate statistical analysis was effective and reasonable lead to an accurate classification of 20 batches of samples from different locations. The results showed that 28 common peaks were observed in the fingerprint and the samples were classified into three clusters. The established method was well validated, and showed high precision, good repeatability, and satisfactory stability. It may serve in the quality control and evaluation of Toosendan Fructus.

Toosendan Fructus  /  HPLC-CAD  /  fingerprint  /  similarity evaluation  /  hierarchical cluster analysis  /  principle components analysis
Chun-ni ZHANG, Ying-zi WANG, Xin-guang SUN, Yang ZHAO, Wei ZHENG, Wen-hua LI, Zhen LONG, Bai-ping MA. Chromatographic fingerprint analysis of Toosendan Fructus by HPLC-CAD coupled with chemometrics methods[J]. Acta Pharmaceutica Sinica, 2017 , 52 (3) : 456 -461 . DOI: 10.16438/j.0513-4870.2016-0965
Appendix
Less
Year 2017 volume 52 Issue 3
PDF
106
32
Cite this Article
BibTeX
Article Info
doi: 10.16438/j.0513-4870.2016-0965
  • Receive Date：2016-09-30
  • Online Date：2026-01-14
  • Published：2017-03-12
Article Data
Affiliations
History
  • Received：2016-09-30
  • Revised：2016-11-23
Funding
Affiliations
    1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
    2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China
    3. Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
    4. ThermoFisher Scientific Corporation, Beijing 100080, China
References
Share
https://castjournals.cast.org.cn/joweb/yxxb/EN/10.16438/j.0513-4870.2016-0965
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House