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GGI as a gene carrier delivering MDR1 siRNA to A549/DDP cells for reversal of multidrug resistance
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Zi-rui WANG, Feng BAI, Xiao-ying ZHANG, Jia-min WU, Ling GUO, Zhi LI, Min FENG*
Acta Pharmaceutica Sinica | 2017, 52(2) : 309 - 317
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Acta Pharmaceutica Sinica | 2017, 52(2): 309-317
• Pharmaceutics •
GGI as a gene carrier delivering MDR1 siRNA to A549/DDP cells for reversal of multidrug resistance
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Zi-rui WANG, Feng BAI, Xiao-ying ZHANG, Jia-min WU, Ling GUO, Zhi LI, Min FENG*
Affiliations
  • School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China
Published: 2017-02-12 doi: 10.16438/j.0513-4870.2016-1033
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This study was designed to reverse multidrug resistance of lung cancer cells by downregulating MDR1 genes through RNA interference (RNAi) technology. A novel biodegradable cationic polymer (PEG-b-PLG-g-PEIs, GGI) was synthesized and characterized by 1H NMR. The particle size and zeta potential were measured by dynamic light scattering (DLS). The cell viability profile of GGI was tested by MTT method with both A549 and A549/DDP cell lines. Flow cytometry (FCM) technology was used to investigate the efficiency and intensity of delivering siRNA to cells by GGI polymer. RT-PCR and Western blot were used to detect the mRNA and P-gp expression after GGI/MDR1 siRNA transfection assay. The sensitivity of cisplatin administration after transfecting GGI/MDR1 siRNA polyplexs was performed with MTT and Annexin V-FITC/PI methods. The results suggest that the particle size and zeta potential of GGI/siRNA were 150-200 nm and 16-28 mV. GGI exhibited a lower cell cytotoxity than PEI 25K and higher efficiency of delivering siRNA, which dramatically decreased the expression of MDR1 mRNA and P-gp of A549/DDP cells and increased much sensitivity to cisplatin in A549/DDP cells. GGI holds a great potential in gene delivery as a novel cationic polymer for further investigation.

gene delivery system  /  RNA interference  /  multidrug resistance  /  MDR1 siRNA  /  A549/DDP cell
Zi-rui WANG, Feng BAI, Xiao-ying ZHANG, Jia-min WU, Ling GUO, Zhi LI, Min FENG. GGI as a gene carrier delivering MDR1 siRNA to A549/DDP cells for reversal of multidrug resistance[J]. Acta Pharmaceutica Sinica, 2017 , 52 (2) : 309 -317 . DOI: 10.16438/j.0513-4870.2016-1033
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doi: 10.16438/j.0513-4870.2016-1033
  • Receive Date：2016-10-24
  • Online Date：2026-01-14
  • Published：2017-02-12
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  • Received：2016-10-24
  • Revised：2016-12-08
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    School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
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Genus		 种数
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Percentage of total
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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