Home Journals Articles Updates About
CN
image
收藏切换
Molecular cloning and prokaryotic expression of a type II ribosome inactivating protein from Polyporus umbellatus
收藏切换
PDF
Meng-meng LIU1, 2, Yong-mei XING1, Shun-xin GUO1, *
Acta Pharmaceutica Sinica | 2017, 52(2) : 327 - 332
Less
收藏切换
Acta Pharmaceutica Sinica | 2017, 52(2): 327-332
• Pharmacognosy •
Molecular cloning and prokaryotic expression of a type II ribosome inactivating protein from Polyporus umbellatus
Full
Meng-meng LIU1, 2, Yong-mei XING1, Shun-xin GUO1, *
Affiliations
  • 1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
  • 2. Institute of Bioinformatics and Medical Engineering, School of Electrical and Information Engineering, Jiangsu University of Technology, Changzhou 213001, China
Published: 2017-02-12 doi: 10.16438/j.0513-4870.2016-0731
Outline
收藏切换

A type Ⅱ ribosome inactivating protein (RIP) gene was cloned from Polyporus umbellatus sclerotia by RT-PCR method. The full open reading frame cDNA sequence of this gene was 873 bp in length and encoded a 290-aa protein with a molecular weight of 32.33 kDa and an isoelectric point of 5.58. Multiple sequence alignment revealed that the deduced amino acids possessed conserved domains of RICIN superfamily protein. A neighbor joining phylogenetic analysis suggests that PuRIP was closely related to RIP in Marasmius oreades. Real time PCR results showed that this gene expressed in all tested tissues of P. umbellatus. Meanwhile, the expression of this gene was significantly up-regulated in the part infected by Armillaria mellea. This result suggested that this PuRIP might played important role with potential biotic stress tolerance of P. umbellatus. Otherwise, we successfully constructed the pET15b-PuRIP plasmid, produced and purified the His-PuRIP fusion protein, which would provide the basic material for polyclonal antibody preparation and gene function research.

Polyporus umbellatus  /  type Ⅱ ribosome inactivating protein  /  gene clone  /  expression analysis  /  protein expression and purification
Meng-meng LIU, Yong-mei XING, Shun-xin GUO. Molecular cloning and prokaryotic expression of a type II ribosome inactivating protein from Polyporus umbellatus[J]. Acta Pharmaceutica Sinica, 2017 , 52 (2) : 327 -332 . DOI: 10.16438/j.0513-4870.2016-0731
Appendix
Less
Year 2017 volume 52 Issue 2
PDF
133
50
Cite this Article
BibTeX
Article Info
doi: 10.16438/j.0513-4870.2016-0731
  • Receive Date：2016-07-25
  • Online Date：2026-01-14
  • Published：2017-02-12
Article Data
Affiliations
History
  • Received：2016-07-25
  • Revised：2016-10-10
Funding
Affiliations
    1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
    2. Institute of Bioinformatics and Medical Engineering, School of Electrical and Information Engineering, Jiangsu University of Technology, Changzhou 213001, China
References
Share
https://castjournals.cast.org.cn/joweb/yxxb/EN/10.16438/j.0513-4870.2016-0731
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House