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Transcriptome-wide identification and expression analysis of 2-ODD gene family in Salvia apiana Jepson
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Xiao-jing QIN1, Jia-dong HU1, Zhen-xi FANG1, Wan-sheng CHEN1, 2, Qing LI2, *
Acta Pharmaceutica Sinica | 2022, 57(12) : 3675 - 3685
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Acta Pharmaceutica Sinica | 2022, 57(12): 3675-3685
• Original Articles •
Transcriptome-wide identification and expression analysis of 2-ODD gene family in Salvia apiana Jepson
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Xiao-jing QIN1, Jia-dong HU1, Zhen-xi FANG1, Wan-sheng CHEN1, 2, Qing LI2, *
Affiliations
  • 1. Research and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • 2. Department of Pharmacy, Second Affiliated Hospital of Naval Medical University, Shanghai 200003, China
Published: 2022-12-12 doi: 10.16438/j.0513-4870.2022-0648
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2-Oxoglutarate/Fe(II)-dependent dioxygenases (2-ODD) play an important role in plant primary and secondary metabolism. Based on the high-throughput sequencing platform Illumina NovaSeq 6000, the transcriptome of Salvia apiana Jepson was sequenced, and the obtained reads were de novo assembled. A total of 38 534 unigenes were obtained from the transcriptome. The assembled unigenes were annotated and 29 982 unigenes were given functional annotations. The 2-ODD genes were identified from the assembled S. apiana transcriptome database by bioinformatics methods, and the genes were analyzed, including the homology of the sequences, physicochemical characteristics, signal peptides, transmembrane domains, subcellular localization, secondary structure and tertiary structure, etc. The evolutionary relationships and the expression patterns of the identified 2-ODD genes were also analyzed. 39 full-length 2-ODD genes were identified from the transcriptome of S. apiana. The average length of these 2-ODD encoding proteins was 320 amino acids, the molecular weight was about 36.00 kDa, and most of them were hydrophilic proteins. Phylogenetic analysis divided these 2-ODD genes into several subfamilies. Gene expression analysis indicated that the 2-ODD genes were expressed in different parts of S. apiana, and the expression level of most genes was much higher in roots than that in leaves. This study can lay a foundation for further study of 2-ODD genes in S. apiana.

Salvia apiana Jepson  /  transcriptome  /  2-oxoglutarate/Fe(II)-dependent dioxygenase  /  bioinformatics  /  expression analysis
Xiao-jing QIN, Jia-dong HU, Zhen-xi FANG, Wan-sheng CHEN, Qing LI. Transcriptome-wide identification and expression analysis of 2-ODD gene family in Salvia apiana Jepson[J]. Acta Pharmaceutica Sinica, 2022 , 57 (12) : 3675 -3685 . DOI: 10.16438/j.0513-4870.2022-0648
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Year 2022 volume 57 Issue 12
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Article Info
doi: 10.16438/j.0513-4870.2022-0648
  • Receive Date：2022-05-26
  • Online Date：2025-12-24
  • Published：2022-12-12
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History
  • Received：2022-05-26
  • Revised：2022-06-15
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Affiliations
    1. Research and Development Center of Chinese Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    2. Department of Pharmacy, Second Affiliated Hospital of Naval Medical University, Shanghai 200003, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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