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Preparation of anti-human TIGIT monoclonal antibody and preliminary study of its biological activity
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Zhen-hua WU, Na LI, Juan CHEN, Mei-zhu JIANG, Yao CHEN, Xiao-fen MEI, Hai-bin WANG*
Acta Pharmaceutica Sinica | 2022, 57(11) : 3310 - 3315
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Acta Pharmaceutica Sinica | 2022, 57(11): 3310-3315
• Original Articles •
Preparation of anti-human TIGIT monoclonal antibody and preliminary study of its biological activity
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Zhen-hua WU, Na LI, Juan CHEN, Mei-zhu JIANG, Yao CHEN, Xiao-fen MEI, Hai-bin WANG*
Affiliations
  • BioRay Pharmaceutical Co., Ltd., Taizhou 318000, China
Published: 2022-11-12 doi: 10.16438/j.0513-4870.2022-0121
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T cell immune receptor with Ig and ITIM domains (TIGIT), a promising new target in cancer immunotherapy, plays a critical role in limiting adaptive and innate immunity against tumors. The extracellular domain of human TIGIT was used to immune BALB/c mice, and a new anti-human TIGIT chimeric antibody (c7D3) was developed. The mice in this study were used in accordance with the international guidelines for the care and use of laboratory animals, and the animal study was approved by the Institutional Animal Ethics Committee of AbMax Biotechnology. The biological activity of c7D3 was studied. The results showed that c7D3 exhibited high affinity for TIGIT and effectively inhibited the interaction between TIGIT and its ligands. Cell-based assays indicated that c7D3 induced strong luciferase signaling in TIGIT/CD155 signaling reporter assay and enhanced cytokine secretion in a T cell stimulation assay. The data showed that c7D3 has high binding affinity and excellent blocking bioactivity, supporting the further advancement for therapeutic application.

TIGIT  /  hybridoma  /  monoclonal antibody  /  chimeric antibody  /  bioactivity
Zhen-hua WU, Na LI, Juan CHEN, Mei-zhu JIANG, Yao CHEN, Xiao-fen MEI, Hai-bin WANG. Preparation of anti-human TIGIT monoclonal antibody and preliminary study of its biological activity[J]. Acta Pharmaceutica Sinica, 2022 , 57 (11) : 3310 -3315 . DOI: 10.16438/j.0513-4870.2022-0121
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doi: 10.16438/j.0513-4870.2022-0121
  • Receive Date：2022-01-26
  • Online Date：2025-12-24
  • Published：2022-11-12
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  • Received：2022-01-26
  • Revised：2022-03-25
Affiliations
    BioRay Pharmaceutical Co., Ltd., Taizhou 318000, China
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小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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