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Rapid identification of chemical constituents in the dried stem bark of Asparagus officinalis L. based on UPLC-Q-TOF-MS/MS
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Rui WEI, Lin-jiao YANG, Xue-mei QIN, Zhen-yu LI*
Acta Pharmaceutica Sinica | 2022, 57(9) : 2839 - 2850
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Acta Pharmaceutica Sinica | 2022, 57(9): 2839-2850
Original Articles
Rapid identification of chemical constituents in the dried stem bark of Asparagus officinalis L. based on UPLC-Q-TOF-MS/MS
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Rui WEI, Lin-jiao YANG, Xue-mei QIN, Zhen-yu LI*
Affiliations
  • Modern Research Center for Traditional Chinese Medicine, the Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China
Published: 2022-09-12 doi: 10.16438/j.0513-4870.2022-0652
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Ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) coupled with a molecular network analysis strategy was used to identify the chemical constituents of the stem bark of two kinds of asparagus. The chemical constituents were identified by determining an accurate molecular weight, the fragmentation pathway, and comparison with the mass spectrometry data from the references. A molecular network was established based on the similarity of MS/MS fragmentation patterns. A total of 107 compounds were identified or tentatively deduced, which included 46 saponins, 13 flavonoids, and 48 other compounds. The chemical compounds identified in the stem bark of white and green asparagus differed greatly: the white asparagus was rich in saponins, while the green asparagus was rich in flavonoids. In conclusion, the chemical constituents of asparagus stem bark were characterized rapidly using UPLC-Q-TOF-MS/MS and molecular network analysis, with 10 compounds and 45 targets determined from the HIT 2.0 herbal ingredients' targets platform. This work will provide a theoretical basis for the resource utilization of asparagus.

asparagus stem bark  /  chemical constituent  /  qualitative analysis  /  UPLC-Q-TOF-MS/MS  /  molecular network
Rui WEI, Lin-jiao YANG, Xue-mei QIN, Zhen-yu LI. Rapid identification of chemical constituents in the dried stem bark of Asparagus officinalis L. based on UPLC-Q-TOF-MS/MS[J]. Acta Pharmaceutica Sinica, 2022 , 57 (9) : 2839 -2850 . DOI: 10.16438/j.0513-4870.2022-0652
Year 2022 volume 57 Issue 9
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doi: 10.16438/j.0513-4870.2022-0652
  • Receive Date:2022-05-26
  • Online Date:2025-12-24
  • Published:2022-09-12
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  • Received:2022-05-26
  • Revised:2022-07-19
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    Modern Research Center for Traditional Chinese Medicine, the Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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