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SNP double peak methods through chloroplast genome profiling for identifying Gentiana crassicaulis and two closely related species
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Na-na GAO1, Liang-hong NI1, *, Zhi-li ZHAO1, *, Dorje Gaawe2
Acta Pharmaceutica Sinica | 2022, 57(8) : 2520 - 2527
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Acta Pharmaceutica Sinica | 2022, 57(8): 2520-2527
• Original Articles •
SNP double peak methods through chloroplast genome profiling for identifying Gentiana crassicaulis and two closely related species
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Na-na GAO1, Liang-hong NI1, *, Zhi-li ZHAO1, *, Dorje Gaawe2
Affiliations
  • 1. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • 2. Tibetan Traditional Medical College, Lhasa 850000, China
Published: 2022-08-12 doi: 10.16438/j.0513-4870.2022-0496
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Gentiana crassicaulis Duthie ex Burk. is one of the plant sources of Gentianae Macrophyllae Radix (QinJiao). Gentiana tibetica King ex Hook. f. and Gentiana robusta King ex Hook. f. are relative species of G. crassicaulis. Due to the large intraspecific morphological variation, G. crassicaulis showed high morphological similarity with G. tibetica and G. robusta. And the distribution area of the three species overlaps to some extent, which makes it difficult to identify them. On the basis of morphological identification, the method of molecular identification of the three species was constructed in this study based on chloroplast genomes. The chloroplast genome of Gentiana tibetica is 148 765bp long, with LSC, SSC and IR 81 163 bp, 17 070 bp and 25 266 bp, respectively. The structure of the three is consistent. The chloroplast genome sequences of G. tibetica and G. crassicaulis are highly similar, and the number of variable sites is 9 (149 267 bp in total). Diagnostic SNP that could effectively identify the three species was screened and verified, and a dual-peak SNP detection method was established for the effective identification of each species and mixed samples. Our study provides basic data for the molecular identification of G. crassicaulis and its related species, and the arrangement of related Tibetan medicine.

Gentiana tibetica  /  Gentiana crassicaulis  /  Gentiana robusta  /  chloroplast genome  /  SNP marker  /  double peak detection
Na-na GAO, Liang-hong NI, Zhi-li ZHAO, Dorje Gaawe. SNP double peak methods through chloroplast genome profiling for identifying Gentiana crassicaulis and two closely related species[J]. Acta Pharmaceutica Sinica, 2022 , 57 (8) : 2520 -2527 . DOI: 10.16438/j.0513-4870.2022-0496
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doi: 10.16438/j.0513-4870.2022-0496
  • Receive Date：2022-04-26
  • Online Date：2025-12-23
  • Published：2022-08-12
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History
  • Received：2022-04-26
  • Revised：2022-05-15
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Affiliations
    1. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    2. Tibetan Traditional Medical College, Lhasa 850000, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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