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Cloning, expression and functional identification of cytochrome P450 reductase gene in Aquilaria sinensis
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Yan HAI1, Ning DING1, Ze-kun ZHANG1, Bei-bei ZHANG1, Yu DU1, Xiao-xue ZHANG1, Na LI1, Xiang-yu GE1, Bo-wen GAO2, She-po SHI1, *
Acta Pharmaceutica Sinica | 2022, 57(8) : 2423 - 2429
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Acta Pharmaceutica Sinica | 2022, 57(8): 2423-2429
• Original Articles •
Cloning, expression and functional identification of cytochrome P450 reductase gene in Aquilaria sinensis
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Yan HAI1, Ning DING1, Ze-kun ZHANG1, Bei-bei ZHANG1, Yu DU1, Xiao-xue ZHANG1, Na LI1, Xiang-yu GE1, Bo-wen GAO2, She-po SHI1, *
Affiliations
  • 1. Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
  • 2. Baotou Medical College, Baotou 014060, China
Published: 2022-08-12 doi: 10.16438/j.0513-4870.2022-0152
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Cytochrome P450 reductase (CPR) is essential for the electron transport chain of cytochrome P450s, playing an indispensable role in electron transfer in vivo. In this study, one cDNA encoding cytochrome P450 reductase (Ascpr1) was identified from the callus of Aquilaria sinensis. Ascpr1 contains an open reading frame of 2 124 bp. The deduced protein is composed of 707 amino acids, with a predicted molecular weight of 78.82 kD. Phylogenetic analysis revealed that AsCPR1 is a type Ⅱ CPR protein closely related to the CPR from Theobroma cacao. Transmembrane prediction using TMHMM 2.0 indicated that the amino acids 52-71 of AsCPR1 comprise a transmembrane region. After truncating of 67 amino acid residues from N-terminal, the truncated AsCPR1 was successfully expressed in E. coli Transetta (DE3). Further purification of the recombinant AsCPR1 by affinity chromatography and determination of the enzymatic activity allowed the reducing ability of AsCPR1 to cytochrome C in vitro. The results pave the way for further study on the synthesis of defensive chemicals involved in P450s and the functions of CPR in self-defense of A. sinensis.

Aquilaria sinensis  /  cytochrome P450 reductase  /  gene cloning  /  agarwood
Yan HAI, Ning DING, Ze-kun ZHANG, Bei-bei ZHANG, Yu DU, Xiao-xue ZHANG, Na LI, Xiang-yu GE, Bo-wen GAO, She-po SHI. Cloning, expression and functional identification of cytochrome P450 reductase gene in Aquilaria sinensis[J]. Acta Pharmaceutica Sinica, 2022 , 57 (8) : 2423 -2429 . DOI: 10.16438/j.0513-4870.2022-0152
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Article Info
doi: 10.16438/j.0513-4870.2022-0152
  • Receive Date：2022-02-08
  • Online Date：2025-12-23
  • Published：2022-08-12
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History
  • Received：2022-02-08
  • Revised：2022-03-04
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Affiliations
    1. Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
    2. Baotou Medical College, Baotou 014060, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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