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Analysis of chloroplast genomes and development of specific DNA barcodes for identifying the original species of Rhei Radix et Rhizoma
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Ran-jun LI1, 2, Li-wei WU2, Tian-yi XIN2, Hai LIAO1, Yu-lin LIN2, Hui YAO2, Jia-yu ZHOU1, *, Jing-yuan SONG2, 3, *
Acta Pharmaceutica Sinica | 2022, 57(5) : 1495 - 1505
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Acta Pharmaceutica Sinica | 2022, 57(5): 1495-1505
• Original Articles •
Analysis of chloroplast genomes and development of specific DNA barcodes for identifying the original species of Rhei Radix et Rhizoma
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Ran-jun LI1, 2, Li-wei WU2, Tian-yi XIN2, Hai LIAO1, Yu-lin LIN2, Hui YAO2, Jia-yu ZHOU1, *, Jing-yuan SONG2, 3, *
Affiliations
  • 1. School of Life and Science, Southwest Jiaotong University, Chengdu 610031, China
  • 2. Key Laboratory of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
  • 3. Engineering Research Center of Chinese Medicine Resource of Ministry of Education, Beijing 100193, China
Published: 2022-05-12 doi: 10.16438/j.0513-4870.2021-1525
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Rhei Radix et Rhizoma is one of the most used medicinal materials in China. Its original species are Rheum palmatum, Rh. tanguticum, and Rh. officinale. Rhei Radix et Rhizoma derived from different original species are significantly different in their active ingredients and pharmacological effects. To develop an accurate, rapid, and specific identification method, we obtained the chloroplast genomes of the three original species by Illumina Novaseq sequencing. We designed specific DNA barcodes from the hypervariable regions, which can accurately identify the three original species. The experimental results showed that the total length of the chloroplast genomes of Rh. tanguticum, Rh. officinale and Rh. palmatum were 161 039 bp, 161 093 bp, and 161 136 bp, respectively. All the three genomes were represented as typical quadripartite structures. A total of 131 genes, including 86 protein-coding genes, 37 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes were identified from each chloroplast genome. Five pairs of primers based on the hypervariable regions were designed to efficiently amplify 42 samples. Results confirmed that five hypervariable regions, rps16-trnQ, psaA-ycf3, psbE-petL, ndhF-rpl32, and trnT-trnL, can be used as specific DNA barcodes for the identification of Rh. tanguticum, Rh. officinale, and Rh. palmatum. These results provided genetic information for further species identification of Rhei Radix et Rhizoma, and improve the safety of this clinical medication as well as standardize the market for Rhei Radix et Rhizoma.

Rhei Radix et Rhizoma  /  chloroplast genome  /  original species identification  /  specific DNA barcode
Ran-jun LI, Li-wei WU, Tian-yi XIN, Hai LIAO, Yu-lin LIN, Hui YAO, Jia-yu ZHOU, Jing-yuan SONG. Analysis of chloroplast genomes and development of specific DNA barcodes for identifying the original species of Rhei Radix et Rhizoma[J]. Acta Pharmaceutica Sinica, 2022 , 57 (5) : 1495 -1505 . DOI: 10.16438/j.0513-4870.2021-1525
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Article Info
doi: 10.16438/j.0513-4870.2021-1525
  • Receive Date：2021-10-22
  • Online Date：2025-12-23
  • Published：2022-05-12
Article Data
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History
  • Received：2021-10-22
  • Revised：2022-02-16
Funding
Affiliations
    1. School of Life and Science, Southwest Jiaotong University, Chengdu 610031, China
    2. Key Laboratory of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
    3. Engineering Research Center of Chinese Medicine Resource of Ministry of Education, Beijing 100193, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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