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Determination of six effective ingredients in Imperatae Rhizoma concentrated granules by ultra-performance liquid chromatography using two internal references
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Yu WANG1, 3, Zheng-cai JU1, Ting ZHANG3, Ya-fu XUE1, Li YANG1, 2, *, Zheng-tao WANG1, 2
Acta Pharmaceutica Sinica | 2022, 57(2) : 467 - 473
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Acta Pharmaceutica Sinica | 2022, 57(2): 467-473
• Original Articles •
Determination of six effective ingredients in Imperatae Rhizoma concentrated granules by ultra-performance liquid chromatography using two internal references
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Yu WANG1, 3, Zheng-cai JU1, Ting ZHANG3, Ya-fu XUE1, Li YANG1, 2, *, Zheng-tao WANG1, 2
Affiliations
  • 1. The MOE Key Laboratory for Standardization of Chinese Medicines and SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • 2. Shanghai R&D Centre for Standardization of Chinese Medicines, Shanghai 201203, China
  • 3. Murad Research Center for Modernized Chinese Medicine, Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Published: 2022-02-12 doi: 10.16438/j.0513-4870.2021-1097
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To simultaneously determine the contents of p-coumaric acid, chlorogenic acid, 5-caffeoylquinic acid, 4-caffeoylquinic acid, caffeic acid and ferulic acid in Imperatae Rhizoma concentrated granules, an ultra-high performance liquid chromatography (UPLC) with two internal references method (TIRM) was established and validated. Chromatographic separation was achieved on a ZORBAX RRHD Eclipse Plus C18 column (2.1 mm×100 mm, 1.8 μm) using 1.7 mmol·L-1 oxalic acid in water and methanol as mobile phase. The flow rate was 0.4 mL·min-1 and the column temperature was set as 35℃. The relative correction factors (RCFs) of caffeic acid and ferulic acid using p-coumaric acid as internal reference were calculated and the RCFs of 4-caffeoylquinic acid and 5-caffeoylquinic acid were calculated using chlorogenic acid as the internal reference. The TIRM was fully validated for linearity, accuracy, repeatability, stability and recovery so that it could be compared with the external standard method (ESM). The RCFs of 5-caffeoylquinic acid, 4-caffeoylquinic acid, caffeic acid, and ferulic acid were 1.069, 1.022, 1.368, and 1.493, respectively. The TIRM and ESM were used to determine the contents of six ingredients in Imperatae Rhizoma concentrated granules from different manufacturers and the variation between results was within acceptable limits. In conclusion, the newly established TIRM allowed simultaneous determination of six ingredients (p-coumaric acid, chlorogenic acid, 5-caffeoylquinic acid, 4-caffeoylquinic acid, caffeic acid, ferulic acid) in Imperatae Rhizoma concentrated granules, providing support for the quality control of this traditional Chinese medicine.

Imperatae Rhizoma concentrated granules  /  p-coumaric acid  /  chlorogenic acid  /  quantitative determination analysis multi-component by a single-marker  /  UPLC
Yu WANG, Zheng-cai JU, Ting ZHANG, Ya-fu XUE, Li YANG, Zheng-tao WANG. Determination of six effective ingredients in Imperatae Rhizoma concentrated granules by ultra-performance liquid chromatography using two internal references[J]. Acta Pharmaceutica Sinica, 2022 , 57 (2) : 467 -473 . DOI: 10.16438/j.0513-4870.2021-1097
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Article Info
doi: 10.16438/j.0513-4870.2021-1097
  • Receive Date：2021-07-26
  • Online Date：2025-12-22
  • Published：2022-02-12
Article Data
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History
  • Received：2021-07-26
  • Revised：2021-10-12
Funding
Affiliations
    1. The MOE Key Laboratory for Standardization of Chinese Medicines and SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    2. Shanghai R&D Centre for Standardization of Chinese Medicines, Shanghai 201203, China
    3. Murad Research Center for Modernized Chinese Medicine, Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
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表12种不同金属材料的力学参数
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Family		 属数
Number of
genus		 种数
Number of
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Percentage of
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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