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Establishment of enzymatic hydrolysis method of active Astragalus polysaccharides APS-Ⅱ and study on the immune activity of oligosaccharides after its degradation
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Ke LI1, 2, 3, 4, *, Lian-jie CUI1, 3, 4, 5, Xiao-xia LI6, Li-xia SHI1, 3, 4, Zhen-yu LI1, 3, 4, Xue-mei QIN1, 3, 4, Yu-guang DU2
Acta Pharmaceutica Sinica | 2021, 56(7) : 1936 - 1944
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Acta Pharmaceutica Sinica | 2021, 56(7): 1936-1944
• Original Articles •
Establishment of enzymatic hydrolysis method of active Astragalus polysaccharides APS-Ⅱ and study on the immune activity of oligosaccharides after its degradation
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Ke LI1, 2, 3, 4, *, Lian-jie CUI1, 3, 4, 5, Xiao-xia LI6, Li-xia SHI1, 3, 4, Zhen-yu LI1, 3, 4, Xue-mei QIN1, 3, 4, Yu-guang DU2
Affiliations
  • 1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
  • 2. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
  • 3. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China
  • 4. Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Shanxi University, Taiyuan 030006, China
  • 5. College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China
  • 6. Shanxi Fruit Industry Work Station, Taiyuan 030001, China
Published: 2021-07-12 doi: 10.16438/j.0513-4870.2021-0148
Outline
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Astragalus polysaccharides are the main immunomodulatory substances in Astragali Radix. The structure of polysaccharides is difficult to accurately determine, which limits the in-depth study of the molecular mechanism of Astragalus polysaccharides in vivo. "Polysaccharide receptor theory" believes that there are one or more oligosaccharide fragment "active centers" in immunologically active polysaccharide molecules. Therefore, the degradation of Astragalus polysaccharides into oligosaccharides and the study of the active centers of polysaccharides at the oligosaccharide level provide new ideas in the study of the structure and mechanism of Astragalus polysaccharides. This article adopts endo-α-1, 4-glucanase enzymatic hydrolysis, and determines the best degradation conditions through single factor test and orthogonal test to degrade the immunologically active polysaccharide APS-Ⅱ (10 kDa component) into oligomers with different degrees of polymerization. Then through the preparation of polyacrylamide gel chromatography and specific immune and non-specific immune cell tests, the immune activity screening of different oligosaccharide components is carried out. The animal welfare and the experimental process in this study follow the requirements of the Animal Ethics Committee of Shanxi University. The results showed that compared with the immunologically active polysaccharide APS-Ⅱ, different oligosaccharide components have obvious differences in different immunological activities. This paper studies the different immunological activities of Astragalus polysaccharides at the level of oligosaccharides, laying a foundation for further elucidating the structure and function of Astragalus polysaccharides, enriching the theory of polysaccharide receptors, and providing new ideas for the development of Astragalus polysaccharides.

Astragalus polysaccharide  /  enzymatic hydrolysis  /  immune activity  /  oligosaccharide  /  polysaccharide receptor theory
Ke LI, Lian-jie CUI, Xiao-xia LI, Li-xia SHI, Zhen-yu LI, Xue-mei QIN, Yu-guang DU. Establishment of enzymatic hydrolysis method of active Astragalus polysaccharides APS-Ⅱ and study on the immune activity of oligosaccharides after its degradation[J]. Acta Pharmaceutica Sinica, 2021 , 56 (7) : 1936 -1944 . DOI: 10.16438/j.0513-4870.2021-0148
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Article Info
doi: 10.16438/j.0513-4870.2021-0148
  • Receive Date：2021-01-28
  • Online Date：2025-12-18
  • Published：2021-07-12
Article Data
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History
  • Received：2021-01-28
  • Revised：2021-04-08
Funding
Affiliations
    1. Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China
    2. Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
    3. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China
    4. Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Shanxi University, Taiyuan 030006, China
    5. College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China
    6. Shanxi Fruit Industry Work Station, Taiyuan 030001, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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