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Determination of insulin lispro in rat plasma by LC-MS/MS and its application in a pharmacokinetics study
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Xiao-chu SUN1, 2, Fei-fei LIN2, Mi-mi WAN2, Yue TONG2, Lu CHANG2, Meng YUAN2, Ying-ying FENG2, Guo-sheng TENG1, *, Jia LIU2, *
Acta Pharmaceutica Sinica | 2021, 56(9) : 2383 - 2388
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Acta Pharmaceutica Sinica | 2021, 56(9): 2383-2388
• Special Reports: Bioanalysis for Drug •
Determination of insulin lispro in rat plasma by LC-MS/MS and its application in a pharmacokinetics study
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Xiao-chu SUN1, 2, Fei-fei LIN2, Mi-mi WAN2, Yue TONG2, Lu CHANG2, Meng YUAN2, Ying-ying FENG2, Guo-sheng TENG1, *, Jia LIU2, *
Affiliations
  • 1. School of Chemistry and Life Sciences, Changchun University of Technology, Changchun 130012, China
  • 2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
Published: 2021-09-12 doi: 10.16438/j.0513-4870.2021-0670
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Compared with human insulin, insulin lispro shows a faster hypoglycemic effect and a higher peak plasma concentration, which can better control postprandial hyperglycemia. In this study, we used a solid phase extraction pretreatment method and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to quantify insulin lispro in rat plasma. Bovine insulin was used as an internal standard. Plasma samples were separated on an ACQUITY UPLC Peptide CSH C18 column (2.1 mm × 50 mm, 1.7 μm) after solid phase extraction. Positive electrospray ionization was performed using multiple reaction monitoring (MRM) with transitions of m/z 1 162.5→217.2 for insulin lispro and m/z 1 157.5→136.0 for insulin bovine (internal standard). The method validation results showed that the linear range was 0.1 ng·mL-1-100 ng·mL-1; intra- and inter-day accuracy and precision met the acceptance criteria for biological sample analysis. The recovery of insulin lispro ranged from 63.1% to 68.1%. The method was applied in a pharmacokinetic study of insulin lispro following a single-dose subcutaneous administration to rats. Animal experiments were approved by the Experimental Animal Ethics Committee of Shanghai Institute of Materia Medica, Chinese Academy of Sciences.

insulin lispro  /  LC-MS/MS  /  pharmacokinetics  /  solid phase extraction
Xiao-chu SUN, Fei-fei LIN, Mi-mi WAN, Yue TONG, Lu CHANG, Meng YUAN, Ying-ying FENG, Guo-sheng TENG, Jia LIU. Determination of insulin lispro in rat plasma by LC-MS/MS and its application in a pharmacokinetics study[J]. Acta Pharmaceutica Sinica, 2021 , 56 (9) : 2383 -2388 . DOI: 10.16438/j.0513-4870.2021-0670
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Article Info
doi: 10.16438/j.0513-4870.2021-0670
  • Receive Date：2021-04-30
  • Online Date：2025-12-18
  • Published：2021-09-12
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  • Received：2021-04-30
  • Revised：2021-07-26
Affiliations
    1. School of Chemistry and Life Sciences, Changchun University of Technology, Changchun 130012, China
    2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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