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Development and validation of antibody sandwich enzyme-linked immunosorbent assay method for quantitation of GeXIVA[1, 2] in plasma of rats and Beagle dogs
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Xiao-yu ZHU1, 2, 3, Mei YUAN1, Su-lan LUO2, 3, Jin-jing CHE1, *
Acta Pharmaceutica Sinica | 2021, 56(9) : 2378 - 2382
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Acta Pharmaceutica Sinica | 2021, 56(9): 2378-2382
• Special Reports: Bioanalysis for Drug •
Development and validation of antibody sandwich enzyme-linked immunosorbent assay method for quantitation of GeXIVA[1, 2] in plasma of rats and Beagle dogs
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Xiao-yu ZHU1, 2, 3, Mei YUAN1, Su-lan LUO2, 3, Jin-jing CHE1, *
Affiliations
  • 1. Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China
  • 2. Medical College of Guangxi University, Nanning 530004, China
  • 3. School of Life Science, Hainan University, Haikou 570228, China
Published: 2021-09-12 doi: 10.16438/j.0513-4870.2021-0696
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GeXIVA[1,2] is a new type of conotoxin recently discovered in the transcriptome of Conus generalis and it is expected to be used clinically as a new type of analgesic. This study established and verified a sandwich enzyme-linked immunosorbent assay method for the marine drug GeXIVA[1,2] in the plasma of rats and Beagle dogs. The mouse monoclonal antibody 4B2 and biotin-labeled rabbit polyclonal antibody 2# were developed. The checkerboard method was used to optimize the antibody pairing concentration, minimum dilution ratio, incubation temperature, and incubation time to establish an antibody sandwich ELISA detection method. Verify the established testing methods. The established ELISA method has a quantitative range of 1.25-80 ng·mL-1 in rat and Beagle plasma. The precision, accuracy, selectivity, specificity, stability, dilution linearity, and hook effect all meet the requirements for biological sample analysis. All the procedures for the animal experiments were approved by the Animal Ethics Committee of the Institute (Permit Number: IACUC-DWZX-2020-698). This method can support the preclinical pharmacokinetic study of the marine drug GeXIVA.

GeXIVA[1, 2]  /  peptide  /  sandwich ELISA  /  bioanalysis  /  methodology validation
Xiao-yu ZHU, Mei YUAN, Su-lan LUO, Jin-jing CHE. Development and validation of antibody sandwich enzyme-linked immunosorbent assay method for quantitation of GeXIVA[1, 2] in plasma of rats and Beagle dogs[J]. Acta Pharmaceutica Sinica, 2021 , 56 (9) : 2378 -2382 . DOI: 10.16438/j.0513-4870.2021-0696
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Article Info
doi: 10.16438/j.0513-4870.2021-0696
  • Receive Date：2021-05-10
  • Online Date：2025-12-18
  • Published：2021-09-12
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History
  • Received：2021-05-10
  • Revised：2021-08-03
Funding
Affiliations
    1. Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China
    2. Medical College of Guangxi University, Nanning 530004, China
    3. School of Life Science, Hainan University, Haikou 570228, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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