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Molecular authentication of the medicinal species of Rhizoma et Radix Heraclei, Radix Angelicae Sinensis, Radix Angelicae Pubescentis and Rhizoma et Radix Notopterygii by integrating ITS2 and its secondary structure
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Meng-di ZHENG1, *, Mi-mi SUN2, Zi-han HE1, Huai-zhu LI3
Acta Pharmaceutica Sinica | 2021, 56(8) : 2289 - 2294
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Acta Pharmaceutica Sinica | 2021, 56(8): 2289-2294
• Original Articles •
Molecular authentication of the medicinal species of Rhizoma et Radix Heraclei, Radix Angelicae Sinensis, Radix Angelicae Pubescentis and Rhizoma et Radix Notopterygii by integrating ITS2 and its secondary structure
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Meng-di ZHENG1, *, Mi-mi SUN2, Zi-han HE1, Huai-zhu LI3
Affiliations
  • 1. School of Pharmacy, Xi'an Medical University, Xi'an 710021, China
  • 2. School of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang 712046, China
  • 3. School of Chemistry and Chemical Engineering, Xianyang Normal University, Xianyang 712000, China
Published: 2021-08-12 doi: 10.16438/j.0513-4870.2021-0108
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This study is to identify Chinese medicinal materials Rhizoma et Radix Heraclei, Angelicae Sinensis, Radix Angelicae Pubescentis and Rhizoma et Radix Notopterygii based on ITS2 and its secondary structure. Total 26 ITS sequences of 7 species were downloaded from GenBank, the ITS2 sequences were annotated by HMMer method. The NJ phylogenetic tree was built by MEGA software, the intraspecific and interspecific K2P genetic distance were analyzed by MEGA as well. The ITS2 secondary structures of all taxa were predicted by ITS2 database. Sequence matrix of primary structure and secondary structure was aligned by 4Sale software. And the profile neighbor joining (PNJ) phylogenetic tree was constructed via the the ProfDistS software based on the distance method. The results show that, the average interspecific genetic distance was far greater than the average intraspecific genetic distance, an obvious barcoding gap was noted among all taxa; NJ tree showed that all species were clustered into seperate branches; each species had different secondary structures; the PNJ tree showed higher resolution than NJ tree. Therefore, ITS2 is suggested to be used as a barcode for distinguishing the original plants of Rhizoma et Radix Heraclei, Radix Angelicae Sinensis, Radix Angelicae Pubescentis and Rhizoma et Radix Notopterygii in this study, this provides some scientific basis for classification and accurate identification of these Chinese medicinal materials.

Chinese medicinal material  /  molecular identification  /  ITS2  /  secondary structure
Meng-di ZHENG, Mi-mi SUN, Zi-han HE, Huai-zhu LI. Molecular authentication of the medicinal species of Rhizoma et Radix Heraclei, Radix Angelicae Sinensis, Radix Angelicae Pubescentis and Rhizoma et Radix Notopterygii by integrating ITS2 and its secondary structure[J]. Acta Pharmaceutica Sinica, 2021 , 56 (8) : 2289 -2294 . DOI: 10.16438/j.0513-4870.2021-0108
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Article Info
doi: 10.16438/j.0513-4870.2021-0108
  • Receive Date：2021-01-20
  • Online Date：2025-12-18
  • Published：2021-08-12
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History
  • Received：2021-01-20
  • Revised：2021-04-02
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Affiliations
    1. School of Pharmacy, Xi'an Medical University, Xi'an 710021, China
    2. School of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang 712046, China
    3. School of Chemistry and Chemical Engineering, Xianyang Normal University, Xianyang 712000, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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