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Interaction study of the antibacterial peptide urumin with the H1N1 HA protein of influenza A virus
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Hong-mei LI1, Wei-juan ZHENG1, Jia-huang LI1, 2, 3, *, Zi-chun HUA1, 2, 3, *
Acta Pharmaceutica Sinica | 2021, 56(9) : 2553 - 2560
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Acta Pharmaceutica Sinica | 2021, 56(9): 2553-2560
• Original Articles •
Interaction study of the antibacterial peptide urumin with the H1N1 HA protein of influenza A virus
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Hong-mei LI1, Wei-juan ZHENG1, Jia-huang LI1, 2, 3, *, Zi-chun HUA1, 2, 3, *
Affiliations
  • 1. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China
  • 2. College of Biopharmaceuticals, China Pharmaceutical University, Nanjing 211198, China
  • 3. High-tech Research Institute of Nanjing University at Changzhou and Jiangsu Target Pharma Laboratories Inc., Changzhou 213164, China
Published: 2021-09-12 doi: 10.16438/j.0513-4870.2021-0483
Outline
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Influenza A virus (H1N1) seriously affects the health of human and disrupts the development of global economic. The antimicrobial peptide urumin specifically binds to the conserved stem of the hemagglutinin (HA) protein of H1N1 virus, but its binding site and the mechanism of action are not clear. In this study, we investigated the possible binding sites and key amino acids for the interaction of urumin with HA protein by molecular docking and enzyme-linked immunosorbent assay (ELISA) experiments, suggesting that HA residues His32 (HA1), Asp19 (HA2), and Trp21 (HA2) are the key residues for the interaction of HA with urumin. Urumin's Arg4, Asn9, and Cys16 were associated with HA protein residues Asp19 (HA2), Trp21 (HA2), His32 (HA1), and Asn53 (HA2) form hydrogen bonding interactions, and Trp12 forms an aromatic π-stacking interaction with His32 (HA1) of HA, these interactions maintain the binding of urumin to HA protein. Wild-type HA and its alanine mutant[alanine substitutions His32 (HA1), Asp19 (HA2), and Trp21 (HA2)] were expressed in 293T cells. ELISA experiments showed that the affinity ability of urumin with HA wild-type was significantly higher than that of HA alanine mutant, suggesting that His32 (HA1), Asp19 (HA2), and Trp21 (HA2) may be the key residues for HA to interact with urumin. This study provides a theoretical and experimental basis for further modification and application of urumin.

urumin peptide  /  H1N1 virus  /  hemagglutinin  /  molecular docking simulation  /  binding site
Hong-mei LI, Wei-juan ZHENG, Jia-huang LI, Zi-chun HUA. Interaction study of the antibacterial peptide urumin with the H1N1 HA protein of influenza A virus[J]. Acta Pharmaceutica Sinica, 2021 , 56 (9) : 2553 -2560 . DOI: 10.16438/j.0513-4870.2021-0483
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Year 2021 volume 56 Issue 9
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Article Info
doi: 10.16438/j.0513-4870.2021-0483
  • Receive Date：2021-04-06
  • Online Date：2025-12-18
  • Published：2021-09-12
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History
  • Received：2021-04-06
  • Revised：2021-05-16
Funding
Affiliations
    1. State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China
    2. College of Biopharmaceuticals, China Pharmaceutical University, Nanjing 211198, China
    3. High-tech Research Institute of Nanjing University at Changzhou and Jiangsu Target Pharma Laboratories Inc., Changzhou 213164, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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