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Inhibitory effect of Qing-Fei-Pai-Du decoction on coronavirus in vitro
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Kun WANG1, 2, Hai-yan YAN1, 2, Shuo WU1, 2, Hui-qiang WANG1, 2, Yu-huan LI1, 2, *, Jian-dong JIANG2, *
Acta Pharmaceutica Sinica | 2021, 56(5) : 1400 - 1408
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Acta Pharmaceutica Sinica | 2021, 56(5): 1400-1408
• Original Articles •
Inhibitory effect of Qing-Fei-Pai-Du decoction on coronavirus in vitro
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Kun WANG1, 2, Hai-yan YAN1, 2, Shuo WU1, 2, Hui-qiang WANG1, 2, Yu-huan LI1, 2, *, Jian-dong JIANG2, *
Affiliations
  • 1. CAMS Key Laboratory of Antiviral Drug Research, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
  • 2. Beijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
Published: 2021-05-12 doi: 10.16438/j.0513-4870.2020-1879
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Qing-Fei-Pai-Du decoction (QFPDD) is a combination of traditional Chinese medicine and plays an important role in the treatment of coronavirus disease 2019 (COVID-19). This study investigated the inhibitory effect of QFPDD on coronavirus replication and antiviral mechanism. The cytotoxicity of QFPDD was determined by PrestoBlue cell viability assay. Quantitive reverse transcription PCR (qRT-PCR) and immunofluorescence assay (IF) were used to detect the inhibitory effects of QFPDD on coronavirus at RNA and protein levels. qRT-PCR was used to detect the adsorption and penetration of coronavirus after QFPDD treatment. The effects of QFPDD on interferon (IFN) and interferon-stimulated genes (ISGs) were also detected by qRT-PCR. The results showed that QFPDD inhibited coronavirus at RNA and protein levels in a dose-dependent manner at non-toxic concentration, and QFPDD targeted in the early stages of coronavirus infection cycle. Preliminary mechanism studies have shown that QFPDD can directly block the virus entry into the cell by inhibiting virus adsorption, and QFPDD can also play an antiviral role by up-regulating the expression of IFN and ISGs. These results indicate QFPDD as a drug potential to treat coronavirus infection.

coronavirus  /  Qing-Fei-Pai-Du decoction  /  interferon  /  entry  /  mechanism
Kun WANG, Hai-yan YAN, Shuo WU, Hui-qiang WANG, Yu-huan LI, Jian-dong JIANG. Inhibitory effect of Qing-Fei-Pai-Du decoction on coronavirus in vitro[J]. Acta Pharmaceutica Sinica, 2021 , 56 (5) : 1400 -1408 . DOI: 10.16438/j.0513-4870.2020-1879
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Article Info
doi: 10.16438/j.0513-4870.2020-1879
  • Receive Date：2020-12-08
  • Online Date：2025-12-18
  • Published：2021-05-12
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  • Received：2020-12-08
  • Revised：2020-12-30
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Affiliations
    1. CAMS Key Laboratory of Antiviral Drug Research, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
    2. Beijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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