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Glaucocalyxin A represses TGF-β1-induced lung fibroblast differentiation by down-regulation of GSK3β/β-catenin signaling
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Han-yue ZHU1, 2, Mei ZHU1, Xi-hua CHEN1, Xiao-gang JIANG1, *
Acta Pharmaceutica Sinica | 2021, 56(11) : 2950 - 2956
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Acta Pharmaceutica Sinica | 2021, 56(11): 2950-2956
• Special Reports: Recent Advances in Visceral Organ Fibrosis and Antifibrotic Drug Discovery •
Glaucocalyxin A represses TGF-β1-induced lung fibroblast differentiation by down-regulation of GSK3β/β-catenin signaling
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Han-yue ZHU1, 2, Mei ZHU1, Xi-hua CHEN1, Xiao-gang JIANG1, *
Affiliations
  • 1. College of Pharmaceutical Sciences, School of Medicine, Soochow University, Suzhou 215000, China
  • 2. Department of Pharmacy, Wuxi No. 2 People's Hospital, Wuxi 214000, China
Published: 2021-11-12 doi: 10.16438/j.0513-4870.2021-0819
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In this study, we investigated the effect and mechanism of glaucocalyxin A on transforming growth factor-β1 (TGF-β1)-induced differentiation of lung fibroblasts by Western blotting, cellular immunofluorescence and collagen gel contraction assays. We monitored the phosphorylation of Smad3, measured extracellular regulated protein kinases (ERK) 1/2 and glycogen synthase kinse3β (Ser9) activity and the level of β-catenin to elucidate the role of glaucocalyxin A. The results show that glaucocalyxin A significantly decreased the expression of α-smooth muscle actin in lung fibroblasts; glaucocalyxin A remarkably reduced the formation of filaments and collagen gel contraction of lung fibroblasts; glaucocalyxin A notably down-regulated the production of fibronectin; glaucocalyxin A did not affect the phosphorylation level of Smad3 and ERK1/2; glaucocalyxin A markedly inhibited the phosphorylation of GSK3β (Ser9) and the levels of β-catenin; a GSK3β (S9A) mutant significantly inhibited lung fibroblast differentiation; and SKL2001, a β-catenin activator, partly reversed the inhibition of lung fibroblast differentiation by glaucocalyxin A. These results suggest that glaucocalyxin A significantly inhibits the differentiation of lung fibroblasts, which is related to the down-regulation of GSK3β/β-catenin signaling.

pulmonary fibrosis  /  glaucocalyxin A  /  lung fibroblast  /  transforming growth factor-β1  /  GSK3β/β-catenin
Han-yue ZHU, Mei ZHU, Xi-hua CHEN, Xiao-gang JIANG. Glaucocalyxin A represses TGF-β1-induced lung fibroblast differentiation by down-regulation of GSK3β/β-catenin signaling[J]. Acta Pharmaceutica Sinica, 2021 , 56 (11) : 2950 -2956 . DOI: 10.16438/j.0513-4870.2021-0819
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Year 2021 volume 56 Issue 11
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Article Info
doi: 10.16438/j.0513-4870.2021-0819
  • Receive Date：2021-06-02
  • Online Date：2025-12-18
  • Published：2021-11-12
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  • Received：2021-06-02
  • Revised：2021-07-01
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    1. College of Pharmaceutical Sciences, School of Medicine, Soochow University, Suzhou 215000, China
    2. Department of Pharmacy, Wuxi No. 2 People's Hospital, Wuxi 214000, China
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表12种不同金属材料的力学参数
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Family		 属数
Number of
genus		 种数
Number of
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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