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Genome-scale identification and expression analysis of MLO genes in Lonicera japonica
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Xiao-li LI1, 2, Li WEI3, Qian LIU1, 2, Jia LI1, 2, Fang ZHANG1, 2, Yong-qing ZHANG1, 2, Gao-bin PU1, 2, *
Acta Pharmaceutica Sinica | 2021, 56(10) : 2867 - 2875
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Acta Pharmaceutica Sinica | 2021, 56(10): 2867-2875
• Original Articles •
Genome-scale identification and expression analysis of MLO genes in Lonicera japonica
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Xiao-li LI1, 2, Li WEI3, Qian LIU1, 2, Jia LI1, 2, Fang ZHANG1, 2, Yong-qing ZHANG1, 2, Gao-bin PU1, 2, *
Affiliations
  • 1. Shandong University of Traditional Chinese Medicine, Jinan 250355, China
  • 2. Shandong Provincial Collaborative Innovation Center for Quality Control and Construction of the Whole Industrial Chain of Traditional Chinese Medicine, Jinan 250355, China
  • 3. Shandong Yingcai University, Jinan 250100, China
Published: 2021-10-12 doi: 10.16438/j.0513-4870.2021-0717
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Twenty-five candidate MLO genes of Lonicera japonica were obtained by whole genome sequencing. Bioinformatics analysis showed that the number of amino acids in the protein family ranged from 137 to 846, the theoretical isoelectric point ranged from 5.02 to 9.50, and it was rich in basic amino acids. One protein did not contain transmembrane domain, and the other proteins ranged from 3 to 10. The results of subcellular localization showed that 21 proteins were located on the cell membrane and 1 protein was located on the chloroplast. Phylogenetic trees were constructed from 133 MLO proteins of Lonicera japonica, Triticum aestivum, Arabidopsis thaliana, Solanum lycopersicum, Nicotiana tabacum, Nicotiana sylvestris and Nicotiana tomentosiformis. The results indicated that MLO family proteins of Lonicera japonica could be divided into five subgroups. Tissue specific analysis showed that the expression of MLO genes in Lonicera japonica had obvious tissue specificity. Among them, 8 genes were highly expressed in leaves, 2 genes in stems and 2 genes in flowers. Four genes were significantly upregulated after inoculation with powdery mildew, among which MLO14 increased the most, which was more than 2 000 times higher than that of the control. In this study, the composition and expression of the MLO gene related to the occurrence of powdery mildew of Lonicera japonica were preliminarily analyzed, which laid a foundation for the further use of MLO as a target gene to develop new germplasm resistant to powdery mildew of Lonicera japonica.

Lonicera japonica  /  MLO protein  /  powdery mildew  /  bioinformatics  /  expression pattern
Xiao-li LI, Li WEI, Qian LIU, Jia LI, Fang ZHANG, Yong-qing ZHANG, Gao-bin PU. Genome-scale identification and expression analysis of MLO genes in Lonicera japonica[J]. Acta Pharmaceutica Sinica, 2021 , 56 (10) : 2867 -2875 . DOI: 10.16438/j.0513-4870.2021-0717
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Article Info
doi: 10.16438/j.0513-4870.2021-0717
  • Receive Date：2021-05-14
  • Online Date：2025-12-18
  • Published：2021-10-12
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  • Received：2021-05-14
  • Revised：2021-06-15
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Affiliations
    1. Shandong University of Traditional Chinese Medicine, Jinan 250355, China
    2. Shandong Provincial Collaborative Innovation Center for Quality Control and Construction of the Whole Industrial Chain of Traditional Chinese Medicine, Jinan 250355, China
    3. Shandong Yingcai University, Jinan 250100, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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