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A new evaluation method of reperfusion after thrombolysis in beagle dogs with acute thrombotic cerebral vessel occlusion
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Peng ZHANG1, Yi TIAN2, Gao-ting MA3, Chao-ran DONG1, Ming-chao WANG1, Jian-ming ZHOU4, Liang CAO4, Hai-bo ZHU*, 1
Acta Pharmaceutica Sinica | 2021, 56(10) : 2797 - 2801
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Acta Pharmaceutica Sinica | 2021, 56(10): 2797-2801
• Original Articles •
A new evaluation method of reperfusion after thrombolysis in beagle dogs with acute thrombotic cerebral vessel occlusion
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Peng ZHANG1, Yi TIAN2, Gao-ting MA3, Chao-ran DONG1, Ming-chao WANG1, Jian-ming ZHOU4, Liang CAO4, Hai-bo ZHU*, 1
Affiliations
  • 1. State Key Laboratory for Bioactive Substances and Functions of Natural Medicines, Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
  • 2. Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China
  • 3. Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China
  • 4. Jiangsu Kanion Pharmaceutical Company, Lianyungang 222067, China
Published: 2021-10-12 doi: 10.16438/j.0513-4870.2021-0193
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We provide a new preclinical pharmacodynamic evaluation method of cerebral thrombolytics using beagle dogs with acute thrombotic cerebrovascular occlusion. An intracranial large vessel occlusion animal model was generated by pushing an autologous thrombus to the left carotid artery under X-ray angiography. The experiments were approved by the Animal Care and Welfare Committee Institute of Materia Medica, China Academy of Medical Sciences (CAMS), Peking Union Medical College. According to the degrees of cerebral blood flow reperfusion, the new method has 0, Ⅰ, Ⅱ (Ⅱa and Ⅱb), Ⅲ levels. Respectively, 0 level represents no blood flow through the area of vascular occlusion; Ⅰ level has low perfusion; Ⅱa level is less than half the infusion; Ⅱb level is more than half perfusion; Ⅲ level is completely recanalization perfusion. Alteplase was used as a positive drug to verify the accuracy of the method. Our results suggest that 100% of dogs reached the level of grade I and below in the model group, while 83% reached the level of grade IIa and above in the alteplase group. Results of a rank sum test showed that alteplase significantly improved the reperfusion of occluded cerebral vessels. We propose a method to evaluate the efficacy of thrombolytic drugs on dogs with middle cerebral artery occlusion.

beagle dog  /  acute thrombotic cerebral vessel occlusion  /  thrombolytic drug  /  ischemia reperfusion injury  /  preclinical evaluation method
Peng ZHANG, Yi TIAN, Gao-ting MA, Chao-ran DONG, Ming-chao WANG, Jian-ming ZHOU, Liang CAO, Hai-bo ZHU. A new evaluation method of reperfusion after thrombolysis in beagle dogs with acute thrombotic cerebral vessel occlusion[J]. Acta Pharmaceutica Sinica, 2021 , 56 (10) : 2797 -2801 . DOI: 10.16438/j.0513-4870.2021-0193
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Article Info
doi: 10.16438/j.0513-4870.2021-0193
  • Receive Date：2021-02-04
  • Online Date：2025-12-18
  • Published：2021-10-12
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History
  • Received：2021-02-04
  • Revised：2021-04-17
Funding
Affiliations
    1. State Key Laboratory for Bioactive Substances and Functions of Natural Medicines, Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
    2. Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China
    3. Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China
    4. Jiangsu Kanion Pharmaceutical Company, Lianyungang 222067, China
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