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Characterization and expression analysis of the transcription factor BcWRKY70 in Bupleurum chinense DC.
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Fei-xue FENG1, Rui-hua LÜ2, Yi-min LI3, 4, *, Ming-ying ZHANG3, Kai WANG1, Yu-bo ZHAO1, Jing GAO3, Liang PENG3, Gang ZHANG3, 4, *
Acta Pharmaceutica Sinica | 2021, 56(11) : 3184 - 3189
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Acta Pharmaceutica Sinica | 2021, 56(11): 3184-3189
• Original Articles •
Characterization and expression analysis of the transcription factor BcWRKY70 in Bupleurum chinense DC.
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Fei-xue FENG1, Rui-hua LÜ2, Yi-min LI3, 4, *, Ming-ying ZHANG3, Kai WANG1, Yu-bo ZHAO1, Jing GAO3, Liang PENG3, Gang ZHANG3, 4, *
Affiliations
  • 1. Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, China
  • 2. College of Medical Technology, Shaanxi University of Chinese Medicine, Xianyang 712046, China
  • 3. College of Pharmacy and Shaanxi Qinling Application Development and Engineering Center of Chinese Herbal Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046, China
  • 4. State Key Laboratory of Research and Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi University of Chinese Medicine, Xianyang 712083, China
Published: 2021-11-12 doi: 10.16438/j.0513-4870.2021-0987
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The plant-specific WRKY transcription factor family is involved in the regulation of the response of plants to various environmental factors and biological stress. To study the role of the BcWRKY70 gene in Bupleurum chinense DC., we cloned the open reading frame (ORF) sequence of the BcWRKY70 gene based on the transcriptome database. Bioinformatics analysis of BcWRKY70 and promoter region analysis was carried out, and real-time qRT-PCR was used to detect the expression pattern of BcWRKY70 in different tissues and under the different hormone treatments. The results show that the BcWRKY70 ORF is 948 bp in length, encoding 316 amino acids with a typical WRKY domain. Phylogenetic analysis showed that BcWRKY70 is closely related to GhWRKY70. Its promoter region contains cis-elements that presumably respond to environmental factors such as drought, methyl jasmonic acid (MeJA), and abscisic acid (ABA). qRT-PCR analyses showed that BcWRKY70 is most highly expressed in roots and can quickly respond to MeJA and ABA induction. This study provides a basis for further research on the molecular mechanism of BcWRKY70 in B. chinense DC. resistance.

Bupleurum chinense DC.  /  WRKY transcription factor  /  cloning  /  expression pattern  /  promoter region
Fei-xue FENG, Rui-hua LÜ, Yi-min LI, Ming-ying ZHANG, Kai WANG, Yu-bo ZHAO, Jing GAO, Liang PENG, Gang ZHANG. Characterization and expression analysis of the transcription factor BcWRKY70 in Bupleurum chinense DC.[J]. Acta Pharmaceutica Sinica, 2021 , 56 (11) : 3184 -3189 . DOI: 10.16438/j.0513-4870.2021-0987
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Article Info
doi: 10.16438/j.0513-4870.2021-0987
  • Receive Date：2021-07-05
  • Online Date：2025-12-18
  • Published：2021-11-12
Article Data
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History
  • Received：2021-07-05
  • Revised：2021-08-12
Funding
Affiliations
    1. Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, China
    2. College of Medical Technology, Shaanxi University of Chinese Medicine, Xianyang 712046, China
    3. College of Pharmacy and Shaanxi Qinling Application Development and Engineering Center of Chinese Herbal Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046, China
    4. State Key Laboratory of Research and Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi University of Chinese Medicine, Xianyang 712083, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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