Home Journals Articles Updates About
CN
image
收藏切换
In vitro and in vivo evaluation of folate-mediated triptolide exosome targeted delivery system against malignant melanoma
收藏切换
PDF
Yong-wei GU1, Liang-di JIANG1, 2, Yue DU2, 3, Ai-xue LI1, 2, Ji-yong LIU1, *
Acta Pharmaceutica Sinica | 2021, 56(12) : 3224 - 3232
Less
收藏切换
Acta Pharmaceutica Sinica | 2021, 56(12): 3224-3232
• Special Reports Ⅰ: Anti-tumor Preparations of Traditional Chinese Medicine •
In vitro and in vivo evaluation of folate-mediated triptolide exosome targeted delivery system against malignant melanoma
Full
Yong-wei GU1, Liang-di JIANG1, 2, Yue DU2, 3, Ai-xue LI1, 2, Ji-yong LIU1, *
Affiliations
  • 1. Department of Pharmacy, Fudan University Shanghai Cancer Center, Shanghai 200032, China
  • 2. College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
  • 3. Department of Pharmacy, Children's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200062, China
Published: 2021-12-12 doi: 10.16438/j.0513-4870.2021-1478
Outline
收藏切换

In this study, exosomes were extracted from human malignant melanoma cell A375. Folic acid (FA) receptor was used as target and triptolide (TPL) was used as model drug to prepare exosome targeted drug delivery system, FA-Exo/TPL. The physicochemical properties and antitumor effect were evaluated in vivo and in vitro. Gradient centrifugation method was applied to collect exosomes. Then, exosome was modified with FA for loading TPL. The particle sizes of the FA-Exo/TPL were about 100 nm with a double-layer membrane structure like a tray. It is characteristic of high encapsulation efficiency and drug loading. In vitro experiments showed that FA-Exo/TPL could be effectively uptaken by A375 cells, thus significantly inhibiting proliferation and promoting apoptosis the cells. In vivo experiment results showed that FA-Exo/TPL could effectively inhibit the growth of tumor tissue, prolong the model mice life cycle, and significantly reduce the systemic toxicity of the free drug, playing a synergistic and toxic role. Animal welfare and experimental procedures follow the regulations of the Animal Ethics Committee of Fudan University Shanghai Cancer Center. This study provides a new strategies and methods for the preparation of TPL against malignant melanoma.

triptolide  /  exosome  /  folic acid  /  targeted drug delivery system  /  malignant melanoma
Yong-wei GU, Liang-di JIANG, Yue DU, Ai-xue LI, Ji-yong LIU. In vitro and in vivo evaluation of folate-mediated triptolide exosome targeted delivery system against malignant melanoma[J]. Acta Pharmaceutica Sinica, 2021 , 56 (12) : 3224 -3232 . DOI: 10.16438/j.0513-4870.2021-1478
Appendix
Less
Year 2021 volume 56 Issue 12
PDF
230
81
Cite this Article
BibTeX
Article Info
doi: 10.16438/j.0513-4870.2021-1478
  • Receive Date：2021-10-14
  • Online Date：2025-12-18
  • Published：2021-12-12
Article Data
Affiliations
History
  • Received：2021-10-14
  • Revised：2021-11-09
Funding
Affiliations
    1. Department of Pharmacy, Fudan University Shanghai Cancer Center, Shanghai 200032, China
    2. College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China
    3. Department of Pharmacy, Children's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200062, China
References
Share
https://castjournals.cast.org.cn/joweb/yxxb/EN/10.16438/j.0513-4870.2021-1478
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House