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Discovery and functional characterization of flavone O-glycosyltransferases in Scutellaria baicalensis
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Bo-yun HAN, Zi-long WANG, Shuang WANG, Min YE*, Xue QIAO*
Acta Pharmaceutica Sinica | 2021, 56(12) : 3345 - 3352
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Acta Pharmaceutica Sinica | 2021, 56(12): 3345-3352
Special Reports Ⅱ: Biosynthesis of Plant Natural Products and Synthetic Biology for Their Production
Discovery and functional characterization of flavone O-glycosyltransferases in Scutellaria baicalensis
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Bo-yun HAN, Zi-long WANG, Shuang WANG, Min YE*, Xue QIAO*
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  • State key Laboratory of Natural and Biomimetic Drugs, Peking University - Yunnan Baiyao International Medical Research Center, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China
Published: 2021-12-12 doi: 10.16438/j.0513-4870.2021-0962
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Huang-Qin is a traditional Chinese medicine with antiviral, antioxidant, and anti-inflammatory activities. Its major bioactive compounds are diverse flavone O-glucuronides and glucosides. Although three flavonoid O-glycosyltransferases have been identified from S. baicalensis, this information is not sufficient to elucidate the structural diversity of flavonoid glycosides. In this study, nine glycosyltransferase candidate genes were discovered from S. baicalensis by BLAST analysis and their functions were characterized after heterologous expression. Three new flavone O-glycosyltransferases were able to catalyze the formation of major compounds in S. baicalensis, including baicalin and wogonoside. These enzymes could also utilize exogenous flavones as sugar acceptors. This work further elucidates biosynthetic pathways for Scutellaria flavonoid O-glycosides.

Scutellaria baicalensis  /  flavonoid O-glycoside  /  glycosyltransferase  /  glycosylation  /  functional characterization
Bo-yun HAN, Zi-long WANG, Shuang WANG, Min YE, Xue QIAO. Discovery and functional characterization of flavone O-glycosyltransferases in Scutellaria baicalensis[J]. Acta Pharmaceutica Sinica, 2021 , 56 (12) : 3345 -3352 . DOI: 10.16438/j.0513-4870.2021-0962
Year 2021 volume 56 Issue 12
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doi: 10.16438/j.0513-4870.2021-0962
  • Receive Date:2021-06-30
  • Online Date:2025-12-18
  • Published:2021-12-12
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  • Received:2021-06-30
  • Revised:2021-09-06
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    State key Laboratory of Natural and Biomimetic Drugs, Peking University - Yunnan Baiyao International Medical Research Center, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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