Home Journals Articles Updates About
CN
image
收藏切换
Optimization of the extraction process for polysaccharides from Cistanche and their molecular weight distribution
收藏切换
PDF
Wen YANG1, Hai-yan YU1, Dong WANG2, Xue-mei ZHOU2, Jian-ping ZHANG2, Lei GAO3, *
Acta Pharmaceutica Sinica | 2021, 56(11) : 3112 - 3117
Less
收藏切换
Acta Pharmaceutica Sinica | 2021, 56(11): 3112-3117
• Original Articles •
Optimization of the extraction process for polysaccharides from Cistanche and their molecular weight distribution
Full
Wen YANG1, Hai-yan YU1, Dong WANG2, Xue-mei ZHOU2, Jian-ping ZHANG2, Lei GAO3, *
Affiliations
  • 1. School of Pharmacy, Inner Mongolia Medical University, Hohhot 010000, China
  • 2. Inner Mongolia Key Laboratory of Chinese and Mongolian Medicines Standard Research, Inner Mongolia Pharmaceutical Inspection Institute, Hohhot 010000, China
  • 3. Inner Mongolia Pharmacovigilance Center, Hohhot 010000, China
Published: 2021-11-12 doi: 10.16438/j.0513-4870.2021-0266
Outline
收藏切换

A simple and efficient method for extracting Cistanche polysaccharides was obtained and used to extract four Cistanche polysaccharides. The molecular weight, distribution and polysaccharide mass fraction of the four Cistanche polysaccharides were measured by High performance size exclusion chromatography-multiangle laser light scatter-refractive index detector (HPSEC-MALLS-RID) and differences between the four Cistanche polysaccharides were determined. The results of a single factor test and an orthogonal test showed that the best process parameters for extracting Cistanche polysaccharides used a mixture of enzyme (4%), a ratio of complex enzyme (cellulase∶pectinase) 1∶1, ultrasonic power at 350 W (40 kHz), for 20 min, enzymolysis temperature of 50 ℃, and pH 6. The average yield of three repeated extractions was 5.14%, the mass fraction was 90.78%, the comprehensive index was 47.96%, and the RSD = 1.51%, indicating good reproducibility. The four Bossica Cistanche polysaccharides are all spherical structures but their molecular weights and distributions differ. The optimized compounded enzyme combined with ultrasonic extraction increases the yield of Cistanche polysaccharides. It is a simple, convenient, economical and environmentally friendly extraction method, which lays the foundation for future polysaccharide research.

Cistanche deserticola polysaccharide  /  complex enzyme  /  molecular weight
Wen YANG, Hai-yan YU, Dong WANG, Xue-mei ZHOU, Jian-ping ZHANG, Lei GAO. Optimization of the extraction process for polysaccharides from Cistanche and their molecular weight distribution[J]. Acta Pharmaceutica Sinica, 2021 , 56 (11) : 3112 -3117 . DOI: 10.16438/j.0513-4870.2021-0266
Appendix
Less
Year 2021 volume 56 Issue 11
PDF
147
56
Cite this Article
BibTeX
Article Info
doi: 10.16438/j.0513-4870.2021-0266
  • Receive Date：2021-06-23
  • Online Date：2025-12-18
  • Published：2021-11-12
Article Data
Affiliations
History
  • Received：2021-06-23
  • Revised：2021-09-15
Funding
Affiliations
    1. School of Pharmacy, Inner Mongolia Medical University, Hohhot 010000, China
    2. Inner Mongolia Key Laboratory of Chinese and Mongolian Medicines Standard Research, Inner Mongolia Pharmaceutical Inspection Institute, Hohhot 010000, China
    3. Inner Mongolia Pharmacovigilance Center, Hohhot 010000, China
References
Share
https://castjournals.cast.org.cn/joweb/yxxb/EN/10.16438/j.0513-4870.2021-0266
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House