Home Journals Articles Updates About
CN
image
收藏切换
Design and synthesis of tetrazine bioorthogonal fluorogenic probes
收藏切换
PDF
Dun-yan SU1, 2, Jie LI1, Li-li PAN1, Hao-xing WU1, Wu-yu MAO1, *
Acta Pharmaceutica Sinica | 2021, 56(4) : 1086 - 1095
Less
收藏切换
Acta Pharmaceutica Sinica | 2021, 56(4): 1086-1095
• Original Articles •
Design and synthesis of tetrazine bioorthogonal fluorogenic probes
Full
Dun-yan SU1, 2, Jie LI1, Li-li PAN1, Hao-xing WU1, Wu-yu MAO1, *
Affiliations
  • 1. Department of Nuclear Medicine, West China Hospital, Sichuan University, Chengdu 610041, China
  • 2. Sichuan Polytechnic Technician College, Chengdu 611130, China
Published: 2021-04-12 doi: 10.16438/j.0513-4870.2020-1897
Outline
收藏切换

Bioorthogonal fluorogenic probes are becoming an ideal tool for live-cell fluorescence imaging. With the tetrazine bioorthogonal fluorogenic probe that displays fluorescence enhancement, the tetrazine plays the dual-role of a bioorthogonal reaction unit and the fluorescence quenching unit. The "off" and "on" states of the fluorescence probe are mainly controlled through inverse electron demand Diels-Alder (IEDDA) bioorthogonal reaction. We designed a series of turn-on tetrazine fluorescent probes with Donor-π-Acceptor (D-π-A) structure to achieve a high signal-to-noise ratio and specificity of fluorescence imaging. This series of probes reacted with the dienophile bicyclononyne, and then generated pyridazine structure in-situ that acted as an electron acceptor, resulting in a new D-π-A effect of fluorescent dyes, turning on the intramolecular charge transfer (ICT) effect. By adjusting the electron-donating groups and the degree of conjugation, tunable fluorescence spectra between 400-647 nm with fluorescence turn-on enhanced up to 500-fold have been achieved. This research lays the foundation for the further optimization of tetrazine bioorthogonal fluorescent probes and their applications in molecular imaging and biomedical fields.

tetrazine  /  bioorthogonal reaction  /  pyridazine  /  turn-on fluorescent probe
Dun-yan SU, Jie LI, Li-li PAN, Hao-xing WU, Wu-yu MAO. Design and synthesis of tetrazine bioorthogonal fluorogenic probes[J]. Acta Pharmaceutica Sinica, 2021 , 56 (4) : 1086 -1095 . DOI: 10.16438/j.0513-4870.2020-1897
Appendix
Less
Year 2021 volume 56 Issue 4
PDF
142
47
Cite this Article
BibTeX
Article Info
doi: 10.16438/j.0513-4870.2020-1897
  • Receive Date：2020-12-10
  • Online Date：2025-12-18
  • Published：2021-04-12
Article Data
Affiliations
History
  • Received：2020-12-10
  • Revised：2021-01-11
Funding
Affiliations
    1. Department of Nuclear Medicine, West China Hospital, Sichuan University, Chengdu 610041, China
    2. Sichuan Polytechnic Technician College, Chengdu 611130, China
References
Share
https://castjournals.cast.org.cn/joweb/yxxb/EN/10.16438/j.0513-4870.2020-1897
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House