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The characteristics of bone disseminated cells of 4T1
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Zhuo WANG1, Xian-jing LI2, Yan LI1, *
Acta Pharmaceutica Sinica | 2024, 59(3) : 608 - 615
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Acta Pharmaceutica Sinica | 2024, 59(3): 608-615
• Original Articles •
The characteristics of bone disseminated cells of 4T1
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Zhuo WANG1, Xian-jing LI2, Yan LI1, *
Affiliations
  • 1. School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
  • 2. State Key Laboratory of Natural Medicine, China Pharmaceutical University, Nanjing 211198, China
Published: 2024-03-12 doi: 10.16438/j.0513-4870.2023-1178
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Based on bone metastasis potential of mouse breast cancer 4T1 cells, the bone disseminated breast tumor cells 4T1 (B-4T1) were acquired through the screening of 6-mercaptopurine. The characteristics of B-4T1 were studied by morphological observation, proliferation assay, expression of epithelial and mesenchymal cell markers detection, transcriptome sequencing, and tumor formation experiments. The results showed that B-4T1 was round and spindle-shaped than primary 4T1 cells, and its proliferation rate was reduced, as well as epithelial cell adhesion molecule (EpCAM) and E-cadherin expression. The transcript level of N-cadherin was increased in the B-4T1, but not vimentin, indicating that B-4T1 had partial epithelial mesenchymal transition. Besides, B-4T1 had higher fatty acid metabolism and better tumor formation capacity. This study lays the experimental foundation for the basic study of metastasis in breast cancer. All animal experiments in this paper were conducted in accordance with the standards of the Animal Ethics Committee of China Pharmaceutical University.

breast cancer  /  bone metastasis  /  disseminated breast tumor cell  /  epithelial mesenchymal transition  /  fatty acid metabolism
Zhuo WANG, Xian-jing LI, Yan LI. The characteristics of bone disseminated cells of 4T1[J]. Acta Pharmaceutica Sinica, 2024 , 59 (3) : 608 -615 . DOI: 10.16438/j.0513-4870.2023-1178
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Article Info
doi: 10.16438/j.0513-4870.2023-1178
  • Receive Date：2023-10-17
  • Online Date：2025-11-28
  • Published：2024-03-12
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  • Received：2023-10-17
  • Revised：2024-01-11
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    1. School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
    2. State Key Laboratory of Natural Medicine, China Pharmaceutical University, Nanjing 211198, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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