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Based on LC-MS technology explored the metabolomics of Agrimonia pilosa intervening in non-small cell lung cancer A549 cells
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Ze-hua TONG1, Wen-jun GUO1, Han-rui ZOU1, Li-wei XU3, Ya-juan XU2, *, Wei-fang WANG1, *
Acta Pharmaceutica Sinica | 2024, 59(3) : 704 - 712
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Acta Pharmaceutica Sinica | 2024, 59(3): 704-712
• Original Articles •
Based on LC-MS technology explored the metabolomics of Agrimonia pilosa intervening in non-small cell lung cancer A549 cells
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Ze-hua TONG1, Wen-jun GUO1, Han-rui ZOU1, Li-wei XU3, Ya-juan XU2, *, Wei-fang WANG1, *
Affiliations
  • 1. Changchun University of Chinese Medicine, Changchun 130117, China
  • 2. Jilin Academy of Chinese Medicine Sciences, Changchun 130012, China
  • 3. Affiliated Hospital of Changchun University of Chinese Medicine, Changchun 130021, China
Published: 2024-03-12 doi: 10.16438/j.0513-4870.2023-0777
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The objective of this study was to analyze the effects on cell viability, apoptosis, and cell cycle of non-small cell lung cancer (NSCLC) A549 cells after intervention with Agrimonia pilosa (AP) and investigate Agrimonia pilosa anti-tumor activity in vitro. Meanwhile, liquid chromatography mass spectrometry (LC-MS) metabolomics technology was used to analyze the changes of cellular metabolites and metabolic pathways. The results of this study will provide a theoretical and experimental basis for investigating the mechanism of the effect of Agrimonia pilosa on non-small cell lung cancer A549 cells. The results showed that the cell nucleus of A549 cells crumpled and apoptosis occurred with the increase of drug concentration. The survival rate of the cells decreased, and the inhibition rate reached 21.5% and 91.74% under the low and high dose conditions, respectively. Lactate dehydrogenase (LDH) content increased (P < 0.05). Metabolomics results showed significant differences in metabolism between groups, thirty-three distinct metabolites including LysoPC(24:0/0:0), LysoPC(17:0/0:0) and PC(O-40:5) were deduced. The pathway enrichment showed that the Agrimonia pilosa plays an anti-tumor role mainly by regulating the metabolism of glycerophosphate and purine in A549 cells, in which the effect on glycerophosphate metabolism pathway was most significant. The results of combined pharmacodynamics suggested that Agrimonia pilosa might induce apoptosis and inhibit the growth of A549 cells by regulating LysoPC(24:0/0:0), LysoPC(17:0/0:0) and PC(O-40:5) metabolites in A549 cells.

Agrimonia pilosa  /  metabolomics  /  A549 cell  /  apoptosis  /  cell cycle
Ze-hua TONG, Wen-jun GUO, Han-rui ZOU, Li-wei XU, Ya-juan XU, Wei-fang WANG. Based on LC-MS technology explored the metabolomics of Agrimonia pilosa intervening in non-small cell lung cancer A549 cells[J]. Acta Pharmaceutica Sinica, 2024 , 59 (3) : 704 -712 . DOI: 10.16438/j.0513-4870.2023-0777
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Year 2024 volume 59 Issue 3
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Article Info
doi: 10.16438/j.0513-4870.2023-0777
  • Receive Date：2023-06-22
  • Online Date：2025-11-28
  • Published：2024-03-12
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History
  • Received：2023-06-22
  • Revised：2023-11-09
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Affiliations
    1. Changchun University of Chinese Medicine, Changchun 130117, China
    2. Jilin Academy of Chinese Medicine Sciences, Changchun 130012, China
    3. Affiliated Hospital of Changchun University of Chinese Medicine, Changchun 130021, China
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表12种不同金属材料的力学参数
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Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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