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Analysis of inorganic elements in different batches of earthworm polypeptides by ICP-MS combined with chemometrics technology
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Hong-liu YANG1, Wei-ting ZHONG2, Yu-shi GUO3, Shu-qi LI1, Jin-chai QI1, Yong-gang LIU1, *, Tao MA1, *
Acta Pharmaceutica Sinica | 2024, 59(4) : 1040 - 1047
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Acta Pharmaceutica Sinica | 2024, 59(4): 1040-1047
Original Articles
Analysis of inorganic elements in different batches of earthworm polypeptides by ICP-MS combined with chemometrics technology
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Hong-liu YANG1, Wei-ting ZHONG2, Yu-shi GUO3, Shu-qi LI1, Jin-chai QI1, Yong-gang LIU1, *, Tao MA1, *
Affiliations
  • 1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102401, China
  • 2. Beijing Tide Pharmaceutical Co., Ltd., Beijing 100176, China
  • 3. Baotou Kundulun District Hospital, Baotou 014010, China
Published: 2024-04-12 doi: 10.16438/j.0513-4870.2023-1187
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To establish a method for determining 26 inorganic elements in earthworm polypeptide and determine the elemental content in different batches of earthworm polypeptide, microwave digestion method was used to pre-treat the samples, and ICP-MS method was used to determine the content of 26 elements in different batches of earthworm polypeptide. The linear relationships of 26 elements were good in the range of 0-1 000 μg·L-1, with R2 greater than 0.999, precision RSD 0.21%-2.71%, repeatability RSD 0.19%-4.69%, stability RSD 0.11%-4.24%, and recovery rates of 82.41%-116.16%. The data was plotted using Origin 2022 software to characterize the distribution of elements content. SPSS 27.0 was used for principal component analysis, and SIMCA 14.1 software was used for OPLS-DA analysis. The results showed that among the 26 elements, the higher content of earthworm polypeptide was K, Na, and Ca, followed by Zn, Fe, Al, B and other trace elements, In, Sc, Co, Pb, Bi and other elements had little or no detectable content, and there were differences in the content of polypeptide in different batches. This study provides a theoretical basis for the production quality control, quality evaluation and drug efficacy application of earthworm polypeptide through the determination and analysis of the elements and content of earthworm polypeptide.

ICP-MS  /  microwave digestion  /  earthworm  /  earthworm polypeptide  /  inorganic element
Hong-liu YANG, Wei-ting ZHONG, Yu-shi GUO, Shu-qi LI, Jin-chai QI, Yong-gang LIU, Tao MA. Analysis of inorganic elements in different batches of earthworm polypeptides by ICP-MS combined with chemometrics technology[J]. Acta Pharmaceutica Sinica, 2024 , 59 (4) : 1040 -1047 . DOI: 10.16438/j.0513-4870.2023-1187
Year 2024 volume 59 Issue 4
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Article Info
doi: 10.16438/j.0513-4870.2023-1187
  • Receive Date:2023-10-20
  • Online Date:2025-11-28
  • Published:2024-04-12
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History
  • Received:2023-10-20
  • Revised:2023-12-20
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Affiliations
    1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102401, China
    2. Beijing Tide Pharmaceutical Co., Ltd., Beijing 100176, China
    3. Baotou Kundulun District Hospital, Baotou 014010, China
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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