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DNA molecular identification of Polyrhachis dives medicinal materials
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Li-dan HE1, Kai-jun ZHAO2, Yun PENG3, Fang HUANG1, Hai-li WANG2, *
Acta Pharmaceutica Sinica | 2023, 58(10) : 3140 - 3146
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Acta Pharmaceutica Sinica | 2023, 58(10): 3140-3146
DNA molecular identification of Polyrhachis dives medicinal materials
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Li-dan HE1, Kai-jun ZHAO2, Yun PENG3, Fang HUANG1, Hai-li WANG2, *
Affiliations
  • 1. School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China
  • 2. Nanjing Zhongshan Pharmaceutical Co., Ltd., Jiangsu Classical Prescriptions Engineering Research Center, Nanjing 210046, China
  • 3. Jiangsu Hongdian Research Institute of Traditional Chinese Medicine Industry Co., LTD., Nanjing Clinical Experience Prescription Modernization Engineering Research Center, Nanjing 210042, China
Published: 2023-10-12 doi: 10.16438/j.0513-4870.2023-0269
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In the study, specific primers were designed based on the CO Ⅰ gene sequence of Polyrhachis dives. By optimizing the genomic DNA extraction method and amplification conditions, we established an efficient, specific, and accurate DNA molecular identification method for Polyrhachis dives. In this method, the length of the target fragment was 294-308 bp, and the other counterfeits had no target bands. In this paper, the specific identification method of the origin of Polyrhachis dives established can be used to identify the medicinal materials of Polyrhachis dives accurately.

Polyrhachis vicina Roger  /  Polyrhachis dives  /  DNA molecular identification  /  specific PCR primers  /  CO Ⅰ gene
Li-dan HE, Kai-jun ZHAO, Yun PENG, Fang HUANG, Hai-li WANG. DNA molecular identification of Polyrhachis dives medicinal materials[J]. Acta Pharmaceutica Sinica, 2023 , 58 (10) : 3140 -3146 . DOI: 10.16438/j.0513-4870.2023-0269
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Article Info
doi: 10.16438/j.0513-4870.2023-0269
  • Receive Date：2023-03-07
  • Online Date：2025-11-21
  • Published：2023-10-12
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  • Received：2023-03-07
  • Revised：2023-04-19
Funding
Affiliations
    1. School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China
    2. Nanjing Zhongshan Pharmaceutical Co., Ltd., Jiangsu Classical Prescriptions Engineering Research Center, Nanjing 210046, China
    3. Jiangsu Hongdian Research Institute of Traditional Chinese Medicine Industry Co., LTD., Nanjing Clinical Experience Prescription Modernization Engineering Research Center, Nanjing 210042, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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