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Simultaneously quantitative analysis of 35 components in gualoupi injection using hydrophilic interaction liquid chromatography tandem mass spectrometry
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Xiang TAO1, 2, Jing-xian ZHANG2, *, Qing HU2, Jian SUN2, Ying DONG3, Jin-guo DING3, Hong YU2, Ying-ying SHEN2, Xiu-hong MAO2, Shen JI1, 2, *
Acta Pharmaceutica Sinica | 2023, 58(5) : 1293 - 1300
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Acta Pharmaceutica Sinica | 2023, 58(5): 1293-1300
• Original Articles •
Simultaneously quantitative analysis of 35 components in gualoupi injection using hydrophilic interaction liquid chromatography tandem mass spectrometry
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Xiang TAO1, 2, Jing-xian ZHANG2, *, Qing HU2, Jian SUN2, Ying DONG3, Jin-guo DING3, Hong YU2, Ying-ying SHEN2, Xiu-hong MAO2, Shen JI1, 2, *
Affiliations
  • 1. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • 2. Shanghai Institute for Food and Drug Control, Shanghai 201203, China
  • 3. SPH No.1 Biochemical & Pharmaceutical Co., Ltd., Shanghai 200240, China
Published: 2023-05-12 doi: 10.16438/j.0513-4870.2022-1123
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A hydrophilic interaction chromatography tandem mass spectrometry method was developed for simultaneous quantification of 35 components in gualoupi injection. The analytes were separated with an ACQUITY XBridge Amide column using 20 mmol·L-1 ammonium formate aqueous solution (pH 3.0) as mobile phase A and 20 mmol·L-1 ammonium formate (pH 3.0)∶acetonitrile (1∶9) as mobile phase B for gradient elution. Mass spectrometry with dynamic multiple reaction monitoring and external standard method were used for quantitative analysis. A total of 35 components were determined in 10 batches of gualoupi injection. The results showed that the 35 compounds had a good linear relationship within their respective concentration ranges with the correlation coefficients (R2 > 0.998 0), the recoveries ranged from 76.6% to 118.5%. The results showed that γ-aminobutyric acid, trigonelline, alanine, threonine, homoserine, citrulline, and leucine were abundant in gualoupi injection, while nicotinamide, methylsuccinic acid, cytosine and choline account for a low percentege. The present study provides an important reference for elucidation of the effective material basis and the improvement of quality standard of gualoupi injection.

gualoupi injection  /  hydrophilic interaction liquid chromatography tandem mass spectrometry  /  dynamic multiple reaction monitoring  /  multi-component quantification
Xiang TAO, Jing-xian ZHANG, Qing HU, Jian SUN, Ying DONG, Jin-guo DING, Hong YU, Ying-ying SHEN, Xiu-hong MAO, Shen JI. Simultaneously quantitative analysis of 35 components in gualoupi injection using hydrophilic interaction liquid chromatography tandem mass spectrometry[J]. Acta Pharmaceutica Sinica, 2023 , 58 (5) : 1293 -1300 . DOI: 10.16438/j.0513-4870.2022-1123
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Article Info
doi: 10.16438/j.0513-4870.2022-1123
  • Receive Date：2022-10-21
  • Online Date：2025-11-21
  • Published：2023-05-12
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History
  • Received：2022-10-21
  • Revised：2023-01-15
Funding
Affiliations
    1. Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    2. Shanghai Institute for Food and Drug Control, Shanghai 201203, China
    3. SPH No.1 Biochemical & Pharmaceutical Co., Ltd., Shanghai 200240, China
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栓菌属 Trametes 5 2.39
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