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Preparation and polarization activity research of astragalus polysaccharide-superparamagnetic iron oxide nanocomposite
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Lin-qing HUANG1, 2, Xin-meng SHI1, 2, Jing-rong WANG3, 4, Ding QU1, 2, Yu-ping LIU1, 2, *, Yan CHEN1, 2, *
Acta Pharmaceutica Sinica | 2023, 58(3) : 779 - 788
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Acta Pharmaceutica Sinica | 2023, 58(3): 779-788
• Original Articles •
Preparation and polarization activity research of astragalus polysaccharide-superparamagnetic iron oxide nanocomposite
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Lin-qing HUANG1, 2, Xin-meng SHI1, 2, Jing-rong WANG3, 4, Ding QU1, 2, Yu-ping LIU1, 2, *, Yan CHEN1, 2, *
Affiliations
  • 1. Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China
  • 2. Multi-component of Traditional Chinese Medicine and Microecology Research Center, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China
  • 3. State Key Laboratory for Quality Research of Chinese Medicines, Macau University of Science and Technology, Macau 999078, China
  • 4. State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, China
Published: 2023-03-12 doi: 10.16438/j.0513-4870.2022-1059
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Size and surface modification are the two key factors affecting the effect of macrophages polarization induced by superparamagnetic iron oxide nanoparticles (SPIONs). The smaller the particle size, the better the polarization effect of SPIONs. Besides, the reasonable SPIONs surface modification method can also be used to enhance the polarization effect. In this study, SPIONs was prepared by solvothermal method and optimized by Box-Benhnken center combination design and response surface method. Furthermore, astragalus polysaccharide-superparamagnetic iron oxide nanocomplex (APS-SPIONs) was successfully constructed by EDC/NHS esterification method. The structure of APS-SPIONs was confirmed by dynamic light scatter and infrared spectrometer, and the contents of iron and polysaccharide were characterized by spectrophotometry. The effect of APS-SPIONs on inducing mouse macrophages RAW264.7 polarization was investigated by flow cytometry. The RAW264.7 macrophages-HepG2 human hepatoma cancer cells Transwell co-culture system was established to investigate APS-SPIONs improve anti-tumor function of macrophages in vitro, and the proliferation activity of APS-SPIONs on RAW264.7 detected by cell counting kit-8 (CCK-8) method. The results showed that the average particle size and zeta potential of APS-SPIONs were (82.93 ± 1.47) nm and (-24.00 ± 0.47) mV. Polysaccharide and Fe content were 8.69% and 7.04%, respectively. APS-SPIONs effectively induced the polarization of RAW264.7 into M1 type in vitro, improving the anti-tumor ability of macrophages in a co-culture system, without effecting the proliferation of macrophages. Our study provides a drug development strategy and preliminary research results to educate macrophages and reshape the tumor immune microenvironment to achieve tumor-killing effects.

magnetic iron oxide nanoparticle  /  astragalus polysaccharide  /  macrophage polarization  /  tumor associated macrophage  /  nanomedicine
Lin-qing HUANG, Xin-meng SHI, Jing-rong WANG, Ding QU, Yu-ping LIU, Yan CHEN. Preparation and polarization activity research of astragalus polysaccharide-superparamagnetic iron oxide nanocomposite[J]. Acta Pharmaceutica Sinica, 2023 , 58 (3) : 779 -788 . DOI: 10.16438/j.0513-4870.2022-1059
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Year 2023 volume 58 Issue 3
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Article Info
doi: 10.16438/j.0513-4870.2022-1059
  • Receive Date：2022-09-15
  • Online Date：2025-11-21
  • Published：2023-03-12
Article Data
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History
  • Received：2022-09-15
  • Revised：2022-10-15
Funding
Affiliations
    1. Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China
    2. Multi-component of Traditional Chinese Medicine and Microecology Research Center, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China
    3. State Key Laboratory for Quality Research of Chinese Medicines, Macau University of Science and Technology, Macau 999078, China
    4. State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510120, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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