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Cloning and functional analysis of IPI gene from Fritillaria unibracteata Hsiao et K. C. Hsia.
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Jiao CHEN1, Si-min SONG1, Jie TANG1, Jin-xiu XIN1, Qian ZHANG1, Hong-jie ZHAO1, Xin CHEN2, Jia-yu ZHOU1, *, Hai LIAO1, *
Acta Pharmaceutica Sinica | 2023, 58(2) : 447 - 453
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Acta Pharmaceutica Sinica | 2023, 58(2): 447-453
• Original Articles •
Cloning and functional analysis of IPI gene from Fritillaria unibracteata Hsiao et K. C. Hsia.
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Jiao CHEN1, Si-min SONG1, Jie TANG1, Jin-xiu XIN1, Qian ZHANG1, Hong-jie ZHAO1, Xin CHEN2, Jia-yu ZHOU1, *, Hai LIAO1, *
Affiliations
  • 1. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China
  • 2. The Third People's Hospital of Chengdu, Chengdu 610014, China
Published: 2023-02-12 doi: 10.16438/j.0513-4870.2022-0925
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An open reading frame (ORF) of isopentenyl-diphosphate delta isomerase gene (FuIPI) was cloned from Fritillaria unibracteata Hsiao et K. C. Hsia. (F. unibracteata). Furthermore, the bioinformatics and functional analyses of FuIPI were performed in this study. The result showed that, the ORF of FuIPI gene was 825 bp, encoding a polypeptide of 274 amino acids in length, with a relative molecular mass of about 31 kD and a theoretical isoelectric point of 5.61. Sequence analysis showed that FuIPI contained conserved structural domains and key residues involved in the catalyzing process. The phylogenetic analysis exhibited that FuIPI was closely related to IPIs of Dendrobium officinale and Musa acuminate. Real-time PCR analysis showed that FuIPI was distributed in different tissues of F. unibracteata, but had the highest transcriptional level in leaves, followed by stems, bulbs, and flowers. Furthermore, the FuIPI protein was successfully expressed in Escherichia coli BL21(DE3). The purified FuIPI protein successfully catalyzed the conversion from isopentenyl diphosphate (IPP) to dimethylallyl pyrophosphate (DMAPP). The above results provided a theoretical basis for further investigation of the molecular role of FuIPI in the biosynthesis of alkaloids.

Fritillaria unibracteata  /  isopentenyl-diphosphate delta isomerase  /  gene cloning  /  heterologous expression  /  in vitro activity
Jiao CHEN, Si-min SONG, Jie TANG, Jin-xiu XIN, Qian ZHANG, Hong-jie ZHAO, Xin CHEN, Jia-yu ZHOU, Hai LIAO. Cloning and functional analysis of IPI gene from Fritillaria unibracteata Hsiao et K. C. Hsia.[J]. Acta Pharmaceutica Sinica, 2023 , 58 (2) : 447 -453 . DOI: 10.16438/j.0513-4870.2022-0925
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Article Info
doi: 10.16438/j.0513-4870.2022-0925
  • Receive Date：2022-07-28
  • Online Date：2025-11-21
  • Published：2023-02-12
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History
  • Received：2022-07-28
  • Revised：2022-08-19
Funding
Affiliations
    1. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China
    2. The Third People's Hospital of Chengdu, Chengdu 610014, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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