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Investigation of preparation process of puerarin-carrying plasma exosomes and effect on permeability in an in vitro blood-brain barrier model
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Qi ZHANG1, 2, Lu-ge HAO1, 2, Xin ZHANG1, 2, Sheng-xiao ZHANG1, 2, jian-dong ZHANG1, 2, Zhen-ao ZHAO3, Wei LI1, 2, *
Acta Pharmaceutica Sinica | 2025, 60(5) : 1534 - 1542
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Acta Pharmaceutica Sinica | 2025, 60(5): 1534-1542
• Original Articles •
Investigation of preparation process of puerarin-carrying plasma exosomes and effect on permeability in an in vitro blood-brain barrier model
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Qi ZHANG1, 2, Lu-ge HAO1, 2, Xin ZHANG1, 2, Sheng-xiao ZHANG1, 2, jian-dong ZHANG1, 2, Zhen-ao ZHAO3, Wei LI1, 2, *
Affiliations
  • 1. Department of Pharmacy, Hebei North University, Zhangjiakou 075000, China
  • 2. Hebei Key Laboratory of Neuropharmacology, Zhangjiakou 075000, China
  • 3. Institute of Microcirculation, Hebei North University, Zhangjiakou 075000, China
Published: 2025-05-12 doi: 10.16438/j.0513-4870.2024-0516
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Plasma exosomes (Pla-Exos) were extracted from rats by ultracentrifugation. The ultracentrifugation method extracted rat Pla-Exos at a speed of 150 000 ×g for 2.5 h. Various purification methods including ultracentrifugation, magnetic bead capture method, and ultrafiltration would be employed to purify Pla-Exos. The Pue-Exos were prepared via sonication-assisted and co-incubation methods. The influence factors and levels of the preparation of plasma exosomes carrying puerarin (Pue-Exos) were investigated by RSM plus CCD method with encapsulation rate as an index. Then the characterization, the stability, and in vitro release of Pue-Exos were determined. The particle sizes of exosomes purified by ultracentrifugation, ultrafiltration, or magnetic bead capture method were all within the range of 30-150 nm. The Western blot results showed that purified Pla-Exos and Pue-Exos contained marker proteins TSG101, CD63, and CD81. The TEM showed that purified Pla-Exos and Pue-Exos exhibited well-defined double-layered membrane vesicle structures. The optimal prescription conditions for preparing Pue-Exos were finally determined as follows: 1 h ultrasound time, 39 W ultrasound power, mass ratio of 10∶1. The prepared Pue-Exos exhibit good stability and sustained release, significantly enhancing the in vitro transpermeability of puerarin across the blood-brain barrier (P < 0.01). This study was approved by the Experimental Animal Ethics Review Committee of Hebei North University, and ethics approval number was HBNV202307012103.

puerarin  /  plasma exosome  /  ultracentrifugation  /  magnetic bead capture method  /  blood-brain barrier
Qi ZHANG, Lu-ge HAO, Xin ZHANG, Sheng-xiao ZHANG, jian-dong ZHANG, Zhen-ao ZHAO, Wei LI. Investigation of preparation process of puerarin-carrying plasma exosomes and effect on permeability in an in vitro blood-brain barrier model[J]. Acta Pharmaceutica Sinica, 2025 , 60 (5) : 1534 -1542 . DOI: 10.16438/j.0513-4870.2024-0516
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Year 2025 volume 60 Issue 5
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Article Info
doi: 10.16438/j.0513-4870.2024-0516
  • Receive Date：2024-05-27
  • Online Date：2025-10-29
  • Published：2025-05-12
Article Data
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History
  • Received：2024-05-27
  • Revised：2024-07-14
Funding
Affiliations
    1. Department of Pharmacy, Hebei North University, Zhangjiakou 075000, China
    2. Hebei Key Laboratory of Neuropharmacology, Zhangjiakou 075000, China
    3. Institute of Microcirculation, Hebei North University, Zhangjiakou 075000, China
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表12种不同金属材料的力学参数
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Family		 属数
Number of
genus		 种数
Number of
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Percentage of
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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