Full
Outline
The dried roots of Phytolacca acinosa Roxb. are widely utilized in traditional Chinese medicine for their potent diuretic, anti-edematous, and detoxifying properties. Esculentosides, the primary bioactive constituents of P. acinosa, exhibit various pharmacological activities, including anti-inflammatory, anti-tumor, and antifungal effects. However, their clinical potential is hampered by low natural abundance and the inherent challenges of chemical synthesis due to their complex structural scaffolds, while synthetic biology represents a promising strategy for the sustainable and green production of esculentoside. However, the biosynthetic pathways remain unclear, and their key functional enzymes have not been reported yet. Herein, through activity-guided enzyme mining and transcriptome analyses, three novel glycosyltransferases (PaUGT2, PaUGT15, and PaUGT25) were functionally characterized from P. acinosa callus. These enzymes were capable of catalyzing the regio-selective glycosylation of 28-COOH of phytolaccagenin. Notably, PaUGT2 could efficiently catalyze 28-O-glycosylation of esculentoside A and esculentoside B, and PaUGT15 and PaUGT25 also exhibited catalytic activities toward these substrates. These findings not only preliminarily clarified the crucial enzymes for 28-O-glycosylation and elucidated the molecular basis for the structural diversity of oleanane-type esculentosides in Phytolacca plants, but also paved the way for further deciphering the biosynthetic pathway and constructing an artificial pathway of these glycosides.
239
112
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
