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Centipeda minima (L.) A. Braun et Aschers. is a traditional medicinal plant. Sesquiterpenoids are its core bioactive components, and sesquiterpene synthases (STSs) are the key enzymes in sesquiterpene biosynthesis. In this study, using C. minima as material, nine full-length candidate CmTPSs genes were screened from published transcriptome data by local Blast alignment combined with phylogenetic analysis. Bioinformatics approaches were used to analyze their sequence features, phylogenetic relationships, and conserved motifs. TBtools was employed to construct gene expression heatmaps for investigating their tissue-specific expression patterns. The catalytic functions of CmTPS1, CmTPS3, CmTPS4, and CmTPS5 were further verified by gene cloning, heterologous expression in Saccharomyces cerevisiae, and GC-MS analysis. Results showed that the nine CmTPSs genes fell into five TPS subfamilies, namely TPS-a, TPS-b, TPS-c, TPS-e/f, and TPS-g, and displayed distinct tissue-specific expression in roots, stems, leaves, and flowers. CmTPS1 and CmTPS4 were specific caryophyllene synthases, while CmTPS3 and CmTPS5 were multifunctional synthases. This study provides key gene resources for elucidating the molecular mechanism of sesquiterpene biosynthesis in C. minima, and offers theoretical and technical support for the efficient production of its bioactive constituents and the development of its medicinal resources.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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