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Outline
Coxsackievirus B3 is a major pathogen responsible for viral myocarditis and is associated with various severe diseases, including dilated cardiomyopathy and pancreatitis. However, no vaccines or specific drugs have been approved for CVB3 infection to date. High content screening technology, which integrates confocal microscopy with multidimensional intelligent image analysis, offers significant advantages for drug screening. In this study, utilizing an EGFP-CVB3 reporter virus, we successfully established a stable and reliable quantitative high-content imaging system for screening anti-CVB3 drugs. This system enables the simultaneous acquisition of multi-parametric data at the single-cell level, including infection rate, cell morphology, and organelle status. The assay was validated using known positive compounds. In conclusion, this system facilitates high-throughput screening of candidate compounds against CVB3 within a single viral life cycle, providing a rapid and sensitive technological tool to accelerate the discovery of anti-CVB3 drugs.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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