Full
Outline
Based on the traditional Chinese medicine theory that "excessive Qi transforms into fire", this study investigates whether overdose of the Qi-tonifying herb Astragalus membranaceus (AM) induces "Shanghuo" and analyzes the related components and underlying mechanisms. Using a mouse model of "Shanghuo" with latent herpes simplex virus type 1 (HSV-1) infection, we systematically observed the general state and behavioral changes of the mice, and detected the expression of virus-related genes and proteins as well as lipid peroxidation-related indicators. The results showed that after intervention with AM and its components, the mice exhibited various "Shanghuo" symptoms, including eye inflammation, increased thirst and water intake, dry stool, and hyperactivity. These symptoms were accompanied by reactivation of latent HSV-1 in the brain, upregulation of lipid peroxidation-related proteins arachidonate 15-lipoxy-genase (ALOX15) and 4-hydroxynonenal (4-HNE), downregulation of cluster of differentiation 117 (CD117; c-Kit) protein expression in intestinal tissue, and elevated levels of the lipid peroxidation product 4-HNE. Further component analysis revealed that AM polysaccharides had a particularly significant impact on behavioral indicators, while low-content AM isoflavones and saponins also reactivated latent HSV-1. In conclusion, various components of AM may synergistically reactivate latent HSV-1 and induce "Shanghuo" potentially through enhancing lipid peroxidation in the body.This experiment has been approved by the Animal Experimental Ethics Committee of Jinan University (IACUC-20250417-04).
208
104
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
