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Outline
The study aims to establish a method for the content determination of eleven components in Jinchai Qingre Granule by high performance liquid chromatography-diode array detector (HPLC-DAD) through quantitative analysis of multi-components by single marker (QAMS) and verify the feasibility and applicability of the method in its quality control. Agilent ZORBAX SB-C18 column (250 mm × 4.6 mm, 5 μm) was used; acetonitrile-0.1% phosphoric acid aqueous solution was used as mobile phase; gradient elution and the detection wavelength were set at 327, 278, 250 nm; flow rate was 1.0 mL·min-1; column temperature was 25 ℃. Using puerarin as the internal reference, the relative correction factors of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, daidzin, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, baicalin, daidzein and arctiin was established, and the eleven components were determined. Eleventeen components showed good linear relationships within their own ranges (r ≥ 0.999 5), whose average recoveries were 98.05%-100.90% with the RSDs of 0.98%-2.35% (n = 6). The method showed good durability under different conditions such as instruments, columns, column temperature, injection volume, and flow rate. There was no significant difference between the calculated values of eleven components in ten batches of samples by the QAMS and the measured values of the external standard method (P > 0.05). The method established in the study is simple and stable, and can be used for the synchronous quality control of eleven index components in Jinchai Qingre Granule.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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