Full
Outline
The genus Fusarium is a prevalent contaminant in medicinal herbs, posing a dual threat to both the effective components and safety of traditional Chinese medicine (TCM), as well as a common pathogen in agriculture and clinical settings. Accurate identification of closely related species within this genus has always been a key challenge. In this study, we employed analysis of whole-GEnome (AGE) to conduct bioinformatics analysis on 304 genomes from 173 species of Fusarium, successfully constructing a species-specific target sequence library. The number of specific target sequences predominantly ranges from 10 001 to 50 000. To validate the reliability of the species-specific target sequences, we selected seven representative species of high concern in contaminated medicinal herbs and agricultural pathogens: Fusarium oxysporum, F. solani, F. fujikuroi, F. sambucinum, F. culmorum, F. incarnatum, and F. equiseti. Species-specific target sequence was selected from each species and subjected to detection using Sanger sequencing and CRISPR-Cas12a technology. The results showed that the species-specific target sequences exhibited high specificity, solving the identification challenge of closely related species F. incarnatum and F. equiseti. Furthermore, AGE enabled the visualization of identification results, offering an advantage for field detection. This study demonstrates that AGE can accurately identify closely related species of the genus Fusarium, providing technical support for the safety monitoring and quality assurance of medicinal herbs.
242
119
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
