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Outline
Influenza A viruses (IAVs) exhibit rapid mutation and high infectivity, leading to a considerable number of cases and deaths globally. Thus, the development of broad-spectrum inhibitors is of paramount significance. In this study, targeting the hemagglutinin (HA) of IAVs, a set of high-affinity and specific aptamers, namely Apt(HA)-1, Apt(HA)-4, Apt(HA)-9, Apt(HA)-25, and Apt(HA)-93520, were obtained through CE-SELEX. The dissociation constants (KD) of this group of aptamers [Apts(HA)] with the HA protein were determined by the SPR method to be 25.9, 44.8, 50.7, 54.0, and 45.2 nmol·L-1, respectively. Apts(HA) can recognize and detect the internationally common influenza virus strains A/PR/8/34 (H1N1) and A/FM/1/47 (H1N1), as well as the clinically isolated Tamiflu-resistant strain A/Liaoningzhenxing/1109/2010 (H1N1), amantadine-resistant strains A/Hunanzhuhui/1222/2010 (H3N2) and A/Fujiantongan/196/2009 (H3N2), and possess broad-spectrum inhibitory effects on influenza viruses. These aptamer inhibitors feature strong in vitro accessibility, good stability, simple structure, and small size. This study not only developed novel broad-spectrum aptamers against IAVs, exerting extensive inhibitory effects on various IAVs, but also paved the way for an effective strategy in the development of universal virus inhibitors.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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