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Structural elucidation of the O-antigen from enteropathogenic Escherichia coli strain EPEC080
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Xin-tong HAN1, 4, Chun-jun QIN1, 4, Guang-zong TIAN1, 4, Xiao-peng ZOU1, 4, Xi GUO5, Jing HU2, 4, *, Jian YIN1, 3, 4, *
Acta Pharmaceutica Sinica | 2025, 60(7) : 2024 - 2034
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Acta Pharmaceutica Sinica | 2025, 60(7): 2024-2034
• Special Reports: Research progress and development trend of carbohydrate-based vaccines •
Structural elucidation of the O-antigen from enteropathogenic Escherichia coli strain EPEC080
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Xin-tong HAN1, 4, Chun-jun QIN1, 4, Guang-zong TIAN1, 4, Xiao-peng ZOU1, 4, Xi GUO5, Jing HU2, 4, *, Jian YIN1, 3, 4, *
Affiliations
  • 1School of Biotechnology and Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China
  • 2Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China
  • 3School of Life Sciences and Health Engineering, Key Laboratory of Carbohydrate Vaccines and Drugs in Jiangsu Province, Jiangnan University, Wuxi 214122, China
  • 4Innovation Center for Vaccine Engineering, Jiangnan University, Wuxi 214122, China
  • 5TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin 300457, China
Published: 2025-07-12 doi: 10.16438/j.0513-4870.2025-0073
Outline
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O-Antigens is the outermost component of bacterial lipopolysaccharide (LPS). Their structural complexity and diversity are essential for bacterial immune evasion, pathogenicity, and bacteria-host interactions. In this study, the O-antigen of lipopolysaccharide from Escherichia coli strain EPEC080 was extracted and purified. The chemical structure of the O-antigen was determined by a combination of various analytical methods, including molecular weight determination, monosaccharide composition analysis, infrared spectrum and nuclear magnetic resonance analysis. The O-antigen with an average molecular weight of 14.3 kDa was determined to consist of a pentasaccharide repeating unit: →4)-[α-D-GalpNAc-(1→3)]-α-L-Rhap-(1→2)-[β-D-GalpNAc-(1→3)]-α-L-Rhap-(1→2)-α-L-Rhap-(1→. Notably, this structure is different from those of known E. coli LPS O-antigens, indicating that strain EPEC080 represents a novel E. coli serotype. The structural identification of this O-antigen provides a foundation for further investigation of the pathogenesis of enteropathogenic E. coli and development of prophylaxis and treatment methods against the related diseases.

enteropathogenic Escherichia coli  /  lipopolysaccharide  /  O-antigen  /  serotype  /  structural elucidation
韩欣童, 秦春君, 田光宗, 邹小鹏, 郭玺, 胡静, 尹健. 肠致病性大肠杆菌EPEC080菌株O-抗原的结构鉴定. 药学学报, 2025 , 60 (7) : 2024 -2034 . DOI: 10.16438/j.0513-4870.2025-0073
Xin-tong HAN, Chun-jun QIN, Guang-zong TIAN, Xiao-peng ZOU, Xi GUO, Jing HU, Jian YIN. Structural elucidation of the O-antigen from enteropathogenic Escherichia coli strain EPEC080[J]. Acta Pharmaceutica Sinica, 2025 , 60 (7) : 2024 -2034 . DOI: 10.16438/j.0513-4870.2025-0073
Appendix
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Year 2025 volume 60 Issue 7
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Article Info
doi: 10.16438/j.0513-4870.2025-0073
  • Receive Date：2025-01-20
  • Online Date：2026-09-20
  • Published：2025-07-12
Article Data
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History
  • Received：2025-01-20
  • Revised：2025-03-09
Affiliations
    1School of Biotechnology and Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China
    2Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China
    3School of Life Sciences and Health Engineering, Key Laboratory of Carbohydrate Vaccines and Drugs in Jiangsu Province, Jiangnan University, Wuxi 214122, China
    4Innovation Center for Vaccine Engineering, Jiangnan University, Wuxi 214122, China
    5TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin 300457, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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